{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T21:10:54Z","timestamp":1759957854295,"version":"build-2065373602"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23792136","20K09878","23K10845"],"award-info":[{"award-number":["23792136","20K09878","23K10845"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Taste sensitivity decreases with age. Therefore, we investigated the histological and immunohistochemical changes in the receptive fields circumvallate papilla (CvP) and fungiform papilla (FfP) to explore the mechanism underlying age\u2010related changes in taste sensitivity in 6\u2010 to 72\u2010week\u2010old rats. We analyzed papilla size, the thickness of the keratin layer of the papilla and stratified squamous epithelium, taste bud size, the keratin layer around the taste pores in the CvP and FfP, and the number and distribution of taste buds in the CvP coronal section. We further assessed the expression of marker proteins for Type II and III cells, phospholipase C subtype beta 2 (PLC\u03b22), and synaptosomal\u2010associated protein 25 (SNAP\u201025). The cellular activity of these taste cells was examined through co\u2010localization with the senescence cell marker protein\u201030 (SMP30). There were no differences in the number of taste bud sections in the CvP among the age groups. However, the size of the CvP increased and the density of the taste bud area in the CvP area decreased with increasing age. In contrast, the number of cells with co\u2010expression of SMP30, PLC\u03b22, and SNAP\u201025 decreased with age. Furthermore, the morphological structures of the CvP, FfP, and taste buds in these regions changed with age, but not the overall taste bud number in the CvP coronal section. The decrease in cell count with co\u2010expression of SMP30 and PLC\u03b22, or SNAP\u201025 may indicate reduced cellular functions of taste cells with aging.<\/jats:p>","DOI":"10.1002\/ar.25338","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T01:08:07Z","timestamp":1697072887000},"page":"414-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Histological and immunohistochemical studies of the fungiform and the circumvallate papillae through the life stages from 6\u2010 to 72\u2010week\u2010old <scp>Sprague\u2010Dawley<\/scp> male rats"],"prefix":"10.1002","volume":"307","author":[{"given":"Fatma M.","family":"Abdel\u2010maksoud","sequence":"first","affiliation":[{"name":"Department of Tissue and Developmental Biology Osaka University Graduate School of Dentistry  Suita Osaka Japan"},{"name":"Department of Anatomy and Embryology, Faculty of Veterinary Medicine Assiut 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