{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T01:05:35Z","timestamp":1759971935149,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T00:00:00Z","timestamp":1698969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inversin\u2010deficient (<jats:italic>inv<\/jats:italic>) mice have anomalies in liver and pancreatic development in addition to an inverted left\u2013right axis of the body. The present study was undertaken to unveil mechanisms of bile and pancreatic duct development from immunohistochemical analyses of anomalies in <jats:italic>inv<\/jats:italic> mice. Intrahepatic bile ducts having proximodistal polarity in size and the height of their epithelia, and ductules were formed in livers of wild\u2010type neonates. By contrast, in <jats:italic>inv<\/jats:italic> mice, ductal plates, precursor structures of intrahepatic bile ducts and ductules, persisted without the proximodistal polarity. Their epithelial cells did not acquire planar cell polarity (PCP) in terms of expression of tight junction proteins although they expressed bile duct markers, HNF1\u03b2 and SOX9. They had an apicobasal polarity from expression of basal laminar components. Enlargement of the hepatic artery and poor connective tissue development, including the abnormal deposition of the extracellular matrices, were also noted in <jats:italic>inv<\/jats:italic> mice, suggesting that bile duct development was coupled to that of the hepatic artery and portal vein. In pancreata of <jats:italic>inv<\/jats:italic> neonates, neither the main pancreatic duct was formed, nor dilated duct\u2010like structures had the morphological polarity from the connecting point with the common bile duct. Lumina of acini was dilated, and centroacinar cells changed their position in the acini to their neck region. Immunohistochemical analyses of tight junction proteins suggested that epithelial cells of the duct\u2010like structures did not have a PCP. Thus, <jats:italic>Invs<\/jats:italic> may be required for the establishment of the PCP of the whole duct system in the liver and pancreas.<\/jats:p>","DOI":"10.1002\/ar.25346","type":"journal-article","created":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T09:20:45Z","timestamp":1699003245000},"page":"2197-2212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inversin\u2010deficient (<i>inv<\/i>) mice do not establish a polarized duct system in the liver and pancreas"],"prefix":"10.1002","volume":"307","author":[{"given":"Hiromu","family":"Fujisawa","sequence":"first","affiliation":[{"name":"Department of Biology, Faculty of Science Shizuoka University  Shizuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9209-4918","authenticated-orcid":false,"given":"Noriaki","family":"Ota","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Shizuoka University  Shizuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6278-3480","authenticated-orcid":false,"given":"Nobuyoshi","family":"Shiojiri","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Shizuoka University  Shizuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,3]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"107","article-title":"Impaired hepatocyte maturation, abnormal expression of biliary transcription factors, and liver fibrosis in C\/EBPa\u2010knockout mice","volume":"29","author":"Akai Y.","year":"2014","journal-title":"Histology and 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