{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T04:29:20Z","timestamp":1783139360046,"version":"3.54.6"},"reference-count":66,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T00:00:00Z","timestamp":1739318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/12716\u20107"],"award-info":[{"award-number":["2021\/12716\u20107"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312325\/2022\u20103"],"award-info":[{"award-number":["312325\/2022\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Although the reproduction of some Boidae snakes has been studied through the macroscopic anatomy of the gonads, without microscopic morphology, there is little precision in describing reproductive cycles, especially for males. The relationship between the reproductive cycle\u2014gametogenesis and reproductive seasonality throughout the year\u2014has yet to be detailed for many Boidae species. We present macroscopic and histological data on the reproductive tracts of both male and viviparous female\n                    <jats:italic>Corallus hortulana<\/jats:italic>\n                    (Linnaeus 1758) (Amazon Tree Boa) from the Brazilian Amazon rainforest. Males present spermiogenesis and spermiation mainly during January and June (wet season to beginning\u2010dry season). Contrary to common assumption, we speculate that sperm production in\n                    <jats:italic>C. hortulana,<\/jats:italic>\n                    even in the tropical Amazon, is likely energetically demanding, influenced by rainfall, given that testicular involution occurred between May and November (most of the driest months of the year). Females present ovarian follicles in processes of vitellogenesis from December to August (wet season and part of the dry season). However, newborns are found in the wild mainly in the wet season, characterizing the cycle of female\n                    <jats:italic>C. hortulana<\/jats:italic>\n                    as seasonal. Unprecedentedly, we present the first microscopic description of the pouch and non\u2010glandular uterus in a Boidae species. The cells that compose the epithelial tissue of the non\u2010glandular uterus have been suggested for maintaining and supporting spermatozoa (sperm storage) in snakes, but we found no evidence that sperm storage occurs in this portion of the oviduct in\n                    <jats:italic>C. hortulana<\/jats:italic>\n                    . This study not only improves our understanding of reproductive biology in a boid, but because several aspects of reproductive cycles are conservative in the family (e.g., season of birth of newborn snakes), it aids in the development of effective conservation policies for endangered species such as\n                    <jats:italic>Corallus cropanii<\/jats:italic>\n                    , the rarest Boidae in the Americas.\n                  <\/jats:p>","DOI":"10.1002\/ar.25634","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T04:24:16Z","timestamp":1739334256000},"page":"3271-3282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Reproductive cycles of the arboreal and viviparous snake\n                    <i>Corallus hortulana<\/i>\n                    (Serpentes, Boidae) from the Brazilian Amazon"],"prefix":"10.1002","volume":"308","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9480-5755","authenticated-orcid":false,"given":"Karina M. P.","family":"Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Toxinologia Aplicada Instituto Butantan  S\u00e3o Paulo Brazil"},{"name":"Laborat\u00f3rio de Ecologia e Evolu\u00e7\u00e3o Instituto Butantan  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edgia","family":"Pizzatto","sequence":"additional","affiliation":[{"name":"Centre for Freshwater Ecosystems La Trobe University  Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Fraz\u00e3o","sequence":"additional","affiliation":[{"name":"Centre for Functional Ecology \u2013 Science for People and the Planet (CFE), TERRA Associate Laboratory, Department of Life Sciences University of Coimbra  Coimbra Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3928-1183","authenticated-orcid":false,"given":"Selma M.","family":"Almeida\u2010Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Ecologia e Evolu\u00e7\u00e3o Instituto Butantan  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ermelinda","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal do Amazonas  Manaus Amazonas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,12]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abramoff M. D.","year":"2004","journal-title":"Biophotonics International"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/1443648"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1655\/0733-1347(2002)016[0001:EOTMSI]2.0.CO;2"},{"key":"e_1_2_8_5_1","first-page":"14","article-title":"Biologia reprodutiva de serpentes: Recomenda\u00e7\u00f5es para a coleta e an\u00e1lise de dados","volume":"3","author":"Almeida\u2010Santos S. M.","year":"2014","journal-title":"Herpetologia Brasileira"},{"key":"e_1_2_8_6_1","first-page":"445","volume-title":"Reproduction in neotropical pitvipers, with emphasis on species of the genus Bothrops","author":"Almeida\u2010Santos S. M.","year":"2002"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/0001-3765202420230949"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ahe.13049"},{"issue":"1","key":"e_1_2_8_9_1","first-page":"89","article-title":"Corallus hortulanus (Amazon tree boa). Timing of reproduction","volume":"41","author":"Bernarde P. S.","year":"2010","journal-title":"Herpetological Review"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1670\/122-02A"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/9781108938891.023"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1590\/1809-4392201801921"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2012.11.027"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3897\/neobiota.78.93788"},{"key":"e_1_2_8_15_1","first-page":"335","article-title":"Mating behavior of Amazon Treeboas, Corallus hortulanus (Squamata: Boidae), on Batatas Island, Piau\u00ed, Brazil","volume":"7","author":"Costa Silva P.","year":"2014","journal-title":"Herpetology Notes"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1201\/b10879-8"},{"key":"e_1_2_8_17_1","volume-title":"Miscellaneous publications \u2013 University of Kansas, Museum of Natural History","author":"Fitch H.","year":"1970"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/bij.12089"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/zoo.21646"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.11606\/issn.2316-9079.v18i1p131-135"},{"key":"e_1_2_8_21_1","unstructured":"Henderson R. W.(2015).Natural history of neotropical treeboas (genusCorallus) edition chimaira frankfurt am main."},{"key":"e_1_2_8_22_1","volume-title":"Histologia B\u00e1sica: Texto e Atlas","author":"Junqueira L. C. U.","year":"2023"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00435-023-00613-8"},{"key":"e_1_2_8_24_1","first-page":"290","volume-title":"Environmental and neuroendocrine control of reproduction in snakes","author":"Krohmer R. W.","year":"2011"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2004.06.015"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/azo.12200"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.033100"},{"issue":"2","key":"e_1_2_8_28_1","first-page":"112","article-title":"New record of Corallus cropanii (Boidae, Boinae): A rare snake from the Vale do Ribeira, State of S\u00e3o Paulo, Brazil","volume":"47","author":"Machado\u2010Filho P. R.","year":"2011","journal-title":"Salamandra"},{"key":"e_1_2_8_29_1","first-page":"1","volume-title":"Clima da Regi\u00e3o Amaz\u00f4nica","author":"Marengo J. A.","year":"2021"},{"key":"e_1_2_8_30_1","first-page":"78","article-title":"Natural history of snakes in forests of the Manaus region, Central Amazonia, Brazil","volume":"6","author":"Martins M.","year":"1998","journal-title":"Herpetological Natural History"},{"key":"e_1_2_8_31_1","first-page":"511","volume-title":"Reproductive cycles of tropical snakes","author":"Mathies T.","year":"2011"},{"key":"e_1_2_8_32_1","volume-title":"Tales of giant snakes: A historical natural history of anacondas and pythons","author":"Murphy J. C.","year":"1997"},{"key":"e_1_2_8_33_1","first-page":"149","article-title":"Characteristics of Amazonian climate: Main features","volume":"1","author":"Nobre C. A.","year":"2009","journal-title":"Geophysical Monograph Series"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.2994\/SAJH-D-19-00120.1"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1997.0065"},{"issue":"4","key":"e_1_2_8_36_1","first-page":"190","article-title":"An\u00e1lise histol\u00f3gica do segmento sexual renal de Sua\u00e7uboia","volume":"30","author":"Pinheiro Y. N. A.","year":"2020","journal-title":"Ci\u00eancia Animal"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1890\/0012-9658(2007)88[359:LATAIS]2.0.CO;2"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.2994\/1808-9798(2007)2[107:REOBSW]2.0.CO;2"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1163\/156853809789647121"},{"key":"e_1_2_8_40_1","unstructured":"R Development Core Team. (2021).R: A language and environment for statistical computing. Retrieved fromhttps:\/\/www.r-project.org"},{"key":"e_1_2_8_41_1","volume-title":"Giant constrictors: Biological and management profiles and an establishment risk assessment for nine large species of pythons, anacondas, and the boa constrictor (No. 2009\u20131202)","author":"Reed R. N.","year":"2009"},{"key":"e_1_2_8_42_1","first-page":"1","article-title":"The sexual segment of the kidney of a tropical rattlesnake, Crotalus durissus (Reptilia, Squamata, Vipedidae), and its relationship to seasonal testicular and androgen cycles","volume":"2021","author":"Resende F. C.","year":"2021","journal-title":"Journal of Morphology"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2013.11.011"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/anbe.2001.1755"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20099"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00435-015-0283-6"},{"key":"e_1_2_8_47_1","first-page":"5","article-title":"Reproductive cycles of male snakes and their relationships with climate and female reproductive cycles","volume":"38","author":"Saint\u2010Girons H. S.","year":"1982","journal-title":"Herpetologica"},{"issue":"3","key":"e_1_2_8_48_1","article-title":"Corallus hortulana (Sua\u00e7uboia)","volume":"51","author":"Santos M. L.","year":"2020","journal-title":"Herpetological Review"},{"key":"e_1_2_8_49_1","first-page":"515","volume-title":"Mating system of male Mojave rattlesnakes (Crotalus scutulatus): Seasonal timing of mating, agonistic behavior, spermatogenesis, sexual segment of the kidney, and plasma sex steroids","author":"Schuett G. W.","year":"2002"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1996.6851"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1071\/ZO9770647"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1071\/ZO9770655"},{"key":"e_1_2_8_53_1","first-page":"249","article-title":"Sexual dimorphism in snakes revised","author":"Shine R.","year":"1994","journal-title":"Copeia"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.2307\/1565722"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7998.1998.tb00045.x"},{"key":"e_1_2_8_56_1","first-page":"347","volume-title":"Female reproductive anatomy: Cloaca, oviduct, and sperm storage","author":"Siegel D. S.","year":"2011"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.11606\/issn.2316-9079.v22i1p75-80"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.21439"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24309"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.zool.2020.125816"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1655\/D-18-00023"},{"key":"e_1_2_8_62_1","unstructured":"Silveira A. L. Prudente A. L. C. Arg\u00f4lo A. J. S. Abrah\u00e3o C. R. Nogueira C. Barbo F. &Guedes T. B.(2021).Corallus cropanii. The IUCN Red List of Threatened Species."},{"issue":"1","key":"e_1_2_8_63_1","first-page":"33","article-title":"Reproductive strategies of the semifossorial snake Atractus zebrinus from the Atlantic Forest","volume":"36","author":"Siqueira L. H. C.","year":"2022","journal-title":"Cuadernos de Herpetolog\u00eda"},{"key":"e_1_2_8_64_1","volume-title":"Kaleidoscopic tree boas: The genus Corallus of tropical America","author":"Stafford P. J.","year":"1996"},{"key":"e_1_2_8_65_1","first-page":"87","article-title":"Corallus hortulanus (Amazon tree boa). Neonate size and timing of reproduction in captivity","volume":"48","author":"Viana P. F.","year":"2017","journal-title":"Herpetological Review"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.10830"},{"issue":"1","key":"e_1_2_8_67_1","first-page":"41","article-title":"Dos nuevos registros de vertebrados en la dieta de Corallus hortulanus (Squamata: Boidae) en el noroeste de la Amazon\u00eda","volume":"31","author":"Y\u00e1nez\u2010Mu\u00f1oz M. H.","year":"2017","journal-title":"Cuadernos de Herpetolog\u00eda"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.25634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T16:00:14Z","timestamp":1762531214000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.25634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,12]]},"references-count":66,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/ar.25634"],"URL":"https:\/\/doi.org\/10.1002\/ar.25634","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,12]]},"assertion":[{"value":"2024-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}