{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:31:03Z","timestamp":1786977063554,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Repair of volumetric muscle loss (VML) lesions tends to be poor, leading to fibrosis and functional loss. Here we investigated the effectiveness of fresh versus cryopreserved autologous adipose tissue transplantation to facilitate repair. Wistar rats were distributed into four experimental groups: normal control, VML lesions, VML lesions treated with a fresh autologous adipose tissue graft, and VML treated with a cryopreserved autologous adipose tissue graft. At 60\u2009days post\u2010intervention, a histological, histochemical, and immunohistochemical analysis was performed to assess changes related to the degree of fibrosis, vascularization, and innervation. These changes were quantified and statistically analyzed. The changes generated by the two types of adipose tissue implanted in the VML lesion were highly similar, with some significant differences favoring the use of cryopreserved adipose tissue. Compared with the VML group, the following outcomes were observed: (1) significant presence of regenerated muscle fibers; (2) significant reduction of fibrosis, albeit with a higher proportion of type III collagen; (3) significant increase in the microvascular pattern; and (4) significant reduction in the number of angulated atrophic muscle fibers and increase in cytoarchitectural changes indicative of reinnervation. This study suggests that autologous adipose tissue transplantation in VML lesions promotes reintegrative processes, facilitating the regeneration and formation of new muscle fibers. Furthermore, the freezing did not diminish outcomes in the CAAT group, highlighting its potential for preservation in tissue banks and applications in regenerative medicine.<\/jats:p>","DOI":"10.1002\/ar.70010","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T04:43:23Z","timestamp":1750826603000},"page":"1406-1421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transplants of fresh and cryopreserved autologous adipose tissue improve reintegrative phenomena in newly formed muscle tissue in the reconstruction of muscle volume loss: A histological evaluation"],"prefix":"10.1002","volume":"309","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9047-192X","authenticated-orcid":false,"given":"Irene","family":"Cantarero","sequence":"first","affiliation":[{"name":"Research Group in Muscle Regeneration, Department of Morphological and Socio\u2010Sanitary Sciences Faculty of Medicine and Nursing, University of Cordoba  Cordoba Spain"},{"name":"Maimonides Institute for Biomedical Research of Cordoba IMIBIC Reina Sof\u00eda University Hospital, University of Cordoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9388-5893","authenticated-orcid":false,"given":"Maria A.","family":"Pe\u00f1a\u2010Toledo","sequence":"additional","affiliation":[{"name":"Research Group in Muscle Regeneration, Department of Morphological and Socio\u2010Sanitary Sciences Faculty of Medicine and Nursing, University of Cordoba  Cordoba Spain"},{"name":"Maimonides Institute for Biomedical Research of Cordoba IMIBIC Reina Sof\u00eda University Hospital, University of Cordoba  Cordoba Spain"},{"name":"Dementia and Multiple Sclerosis Unit, Neurology Service Reina Sofia University Hospital  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9770-1844","authenticated-orcid":false,"given":"Maria E.","family":"Lopez\u2010Espejo","sequence":"additional","affiliation":[{"name":"Research Group in Muscle Regeneration, Department of Morphological and Socio\u2010Sanitary Sciences Faculty of Medicine and Nursing, University of Cordoba  Cordoba Spain"},{"name":"Department of Neurosurgery and Clinical Neurophysiology Reina Sofia University Hospital  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jes\u00fas","family":"Gil\u2010Belmonte","sequence":"additional","affiliation":[{"name":"Research Group in Muscle Regeneration, Department of Morphological and Socio\u2010Sanitary Sciences Faculty of Medicine and Nursing, University of Cordoba  Cordoba Spain"},{"name":"Department of Pathology Torrecardenas University Hospital  Almeria Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5600-3276","authenticated-orcid":false,"given":"Rafael","family":"Villalba","sequence":"additional","affiliation":[{"name":"Sectoral Tissue Bank Tissue Establishment of the Center for Transfusion, Tissues and Cells  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5095-6962","authenticated-orcid":false,"given":"Ignacio","family":"Jimena","sequence":"additional","affiliation":[{"name":"Research Group in Muscle Regeneration, Department of Morphological and Socio\u2010Sanitary Sciences Faculty of Medicine and Nursing, University of Cordoba  Cordoba Spain"},{"name":"Maimonides Institute for Biomedical Research of Cordoba IMIBIC Reina Sof\u00eda University Hospital, University of Cordoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2637-1112","authenticated-orcid":false,"given":"Jos\u00e9","family":"Pe\u00f1a\u2010Amaro","sequence":"additional","affiliation":[{"name":"Research Group in Muscle Regeneration, Department of Morphological and Socio\u2010Sanitary Sciences Faculty of Medicine and Nursing, University of Cordoba  Cordoba Spain"},{"name":"Maimonides Institute for Biomedical Research of Cordoba IMIBIC Reina Sof\u00eda University Hospital, University of Cordoba  Cordoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-018-0027-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00061.2007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.101.3.735"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.27365"},{"key":"e_1_2_11_6_1","article-title":"Freeze\u2010dried porous collagen scaffolds for the repair of volumetric muscle loss injuries","volume":"3","author":"Basurto I. M.","year":"2024","journal-title":"bioRxiv"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25042356"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering7030085"},{"key":"e_1_2_11_9_1","volume-title":"Pathology of skeletal muscle","author":"Carpenter S.","year":"2001"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/20417314231201231"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2023.1172740"},{"key":"e_1_2_11_12_1","first-page":"83","volume-title":"Muscle pathology","author":"Chou S. M.","year":"1984"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2013.09.027"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00253"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-020-0096-1"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13062-023-00399-1"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.23841"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12891-022-05760-5"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2021.11.032"},{"key":"e_1_2_11_20_1","volume-title":"Muscle biopsy: A practical approach","author":"Dubowitz V. A.","year":"2021"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13539-011-0041-7"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells9051297"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/41.2.7678272"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13080693"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900626"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2016.08.019"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-018-0922-1"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202300134"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147198"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.24058"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-23913-3"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-023-00313-3"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP282292"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23243"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S101955"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/nan.12853"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12134214"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22136689"},{"key":"e_1_2_11_39_1","first-page":"457","article-title":"Histology of skeletal muscle reconstructed by means of the implantation of autologous adipose tissue: An experimental study","volume":"35","author":"Leiva\u2010Cepas F.","year":"2020","journal-title":"Histology and Histopathology"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v13.i11.1747"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2024.122529"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfmk9040188"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2022.136879"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2015.02.004"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-018-2955-2"},{"key":"e_1_2_11_46_1","first-page":"2","article-title":"Neuromuscular disease: 2023 update","volume":"4","author":"Margeta M.","year":"2023","journal-title":"Free Neuropathology"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2019.0126"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24043564"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13770-020-00288-y"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1078\/0344-0338-00004"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.24105"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241612545"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering7040118"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.24738"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2021.0187"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35524-7"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00009.2007"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023403928385"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.22794"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3008085"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490200113"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00049.2021"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jelekin.2013.07.009"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.2213"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499453"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1185-7"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.508"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.26654"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118635469.ch5"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1177\/1090820X13501690"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.34133\/bmr.0174"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1008937"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2023.06.009"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.15655"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093302"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.70010","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.70010","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.70010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T08:38:57Z","timestamp":1784882337000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.70010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,25]]},"references-count":74,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/ar.70010"],"URL":"https:\/\/doi.org\/10.1002\/ar.70010","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,25]]},"assertion":[{"value":"2025-02-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}