{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:01:23Z","timestamp":1791392483596,"version":"4.3.3"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T00:00:00Z","timestamp":1766534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Research on gonadal development including sexual differentiation of gonads in chelonians is essential for understanding the mechanisms that determine offspring sex and, consequently, the sex ratio of egg clutches. This directly impacts population structure, as sex ratios influence reproductive dynamics and species' long\u2010term viability. The aim of this study was to characterize the gonadal development of scorpion mud\u2010turtle (\n                    <jats:italic>Kinosternon scorpioides<\/jats:italic>\n                    ). A total of 85 embryos from stages 12\u201326 were analyzed, kept under digital temperature control (28.5\u2009\u00b1\u20090.5\u00b0C). The reproductive system was dissected and processed for histological analysis using light and transmission electron microscopy. The average incubation time was 117.35\u2009\u00b1\u20099.5\u2009days. From stage 12 onwards, gonadal ridges containing primordial germ cells (PGCs) were observed. At stage 14, the undifferentiated gonads consisted of cuboidal coelomic epithelial cells that invaginated to form cord\u2010like structures containing germ cells. At stage 20, at approximately 50\u2009days of incubation, sexual differentiation of gonads was noted. The ovaries showed a cortical region containing oogonia and oocytes, and a medullary region with a stroma rich in vessels. The testes were characterized by a peripheral band of dense connective tissue beneath the tunica albuginea, and by the presence of seminiferous tubules occupying the central portion of the organ. The results of this study provide important information on the gonadal development of\n                    <jats:italic>K. scorpioides<\/jats:italic>\n                    , as the identification of the moment of gonadal differentiation, stage 20, makes it possible to understand the influence of the environment on sex determination. These findings provide support for the reproductive management of\n                    <jats:italic>K. scorpioides<\/jats:italic>\n                    and can be applied directly to the sexual control of populations in situ and ex situ.\n                  <\/jats:p>","DOI":"10.1002\/ar.70116","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T11:20:56Z","timestamp":1766575256000},"page":"3455-3466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Gonadal development in scorpion mud\u2010turtles,\n                    <i>Kinosternon scorpioides<\/i>\n                    , in a controlled environment"],"prefix":"10.1002","volume":"309","author":[{"given":"Brenda","family":"Braga","sequence":"first","affiliation":[{"name":"Animal Science Graduate Program Federal University of Par\u00e1  Castanhal Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f4nica","family":"Oliveira\u2010Bahia","sequence":"additional","affiliation":[{"name":"Biological Sciences Institute, Federal University of Par\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Auxiliadora","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Biological Sciences Institute, Federal University of Par\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Ribamar Felipe","family":"Marques","sequence":"additional","affiliation":[{"name":"Brazilian Agricultural Research Corporation (Embrapa) Eastern Amazon  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diva Ara\u00fajo","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Animal Science Graduate Program Federal University of Par\u00e1  Castanhal Par\u00e1 Brazil"},{"name":"Biological Sciences Institute, Federal University of Par\u00e1  Bel\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15515-w"},{"key":"e_1_2_9_3_1","first-page":"32","article-title":"Panorama da Quelonicultura no Brasil\u2014uma estrat\u00e9gia para conserva\u00e7\u00e3o das esp\u00e9cies e gera\u00e7\u00e3o de renda","volume":"21","author":"Andrade P. C. M.","year":"2021","journal-title":"Aquaculture Brasil"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24578"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00435-021-00517-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.therwi.2024.100094"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/411613"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402180315"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.21071\/az.v66i255.2515"},{"key":"e_1_2_9_10_1","unstructured":"deCastro A. B.(2006).Biologia reprodutiva e crescimento do mu\u00e7u\u00e3 Kinosternon scorpioides (Linnaeus 1776) em cativeiro. PhD thesis Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Ci\u00eancia Animal at Universidade Federal do Par\u00e1."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12030358"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2019.2078"},{"key":"e_1_2_9_13_1","unstructured":"Embrapa (Empresa Brasileira de Pesquisa Agropecu\u00e1ria). (1997).Manual de m\u00e9todos de an\u00e1lises de solo Rio de Janeiro: Centro Nacional de Levantamento e Conserva\u00e7\u00e3o do SoloEmbrapa Solos (pp.7\u20138)."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.03.055"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.2307\/1446248"},{"key":"e_1_2_9_16_1","first-page":"21","volume-title":"Turtle sex\u2010determining modes and TSD patterns, and some TSD pattern correlates","author":"Ewert M. 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