{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T05:21:55Z","timestamp":1773897715019,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>After hind limb amputation in lizards, scars or short outgrowths are formed. FGFs may stimulate regeneration. Injections of FGF\u20101\/\u20102 into the limb stump were made until 26\u2009days post\u2010amputation. This treatment gives rise to outgrowths of 1.5\u20133.5\u2009mm within 40\u201370\u2009days post\u2010amputation, containing axial cartilages continuous with stump bones but missing muscles. Immunohistochemical localization in the outgrowth shows 5BrdU\u2010labeled proliferating cells in the apical wound epidermis and perichondrium at 40\u201350\u2009days post\u2010amputation. Low to no proliferation is present in the axial cartilages and connective tissues at 60 and 70\u2009days when growth terminates, except in scales. Immunodetection of FGF8 and Shh shows that the proteins are present at 30\u201340\u2009days in the wound epidermis, apical connective tissue and perichondrium. These proteins lower to disappear in outgrowths from 50 to 70\u2009days post\u2010amputation, coincident with lowering of cell proliferation. The study suggests that the injection of FGF1\/2 initially stimulates the growth of the limb mainly in cartilaginous axial structures, dense connective tissue, and new scales. No stimulation of muscle tissues is observed. Short cartilaginous rods of tibia, fibula or femur are produced but no autopodial elements. The presence of FGF8 and Shh in the wound epidermis and perichondrium of the initial outgrowths and their subsequent downfall reflect the initial growth and the later growth cessation. In future experiments, longer treatments with FGFs, hyaluronate and other signaling proteins, micro\u2010injected in specific regions of the outgrowths may further enhance limb growth in this or other amniote models of limb regeneration.<\/jats:p>","DOI":"10.1002\/ar.70191","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:26:24Z","timestamp":1773887184000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Induction of limb regeneration in lizards in relation to the expression of the signaling proteins\n                    <scp>FGF8<\/scp>\n                    and Shh"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8247-2217","authenticated-orcid":false,"given":"Lorenzo","family":"Alibardi","sequence":"first","affiliation":[{"name":"Comparative Histolab  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.26496\/bjz.2012.134","article-title":"Observations on FGFs immunoreactivity in the regenerating tail blastema and in the limb and tail scars of lizard suggests that FGFs are required for regeneration","volume":"142","author":"Alibardi L.","year":"2012","journal-title":"Belgian Journal of Zoology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.aanat.2016.03.010","article-title":"Immunolocalization of FGF8\/10 in the apical epidermal Peg and blastema of the regenerating tail in lizard marks this apical growing area","volume":"206","author":"Alibardi L.","year":"2016","journal-title":"Annals of Anatomy"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1002\/jez.b.22754","article-title":"Review: Biological and molecular differences between tail regeneration and limb scarring in lizard, an inspiring model addressing limb regeneration in amniotes","volume":"328","author":"Alibardi L.","year":"2017","journal-title":"Journal of Experimental Zoology"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3390\/jfmk2030025","article-title":"FGFs treatments on amputated limbs stimulate the regeneration of long bones, opening new avenues for limb regeneration in amniotes. 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