{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T03:10:55Z","timestamp":1779333055499,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010324","name":"Secretar\u00eda General de Ciencia y T\u00e9cnica, Universidad Nacional del Nordeste","doi-asserted-by":"publisher","award":["16F0014"],"award-info":[{"award-number":["16F0014"]}],"id":[{"id":"10.13039\/501100010324","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Bats have evolved a wide variety of dietary strategies. These diverse diets have driven significant morphological specialization of their tongues. This study focuses on the external and histological morphology of lingual papillae in three South American insectivorous bat species belonging to the family Molossidae:\n                    <jats:italic>Molossus fluminensis<\/jats:italic>\n                    ,\n                    <jats:italic>Molossus molossus<\/jats:italic>\n                    , and\n                    <jats:italic>Eumops patagonicus<\/jats:italic>\n                    . Using scanning electron microscopy (SEM), conventional histological techniques, and immunohistochemistry, we identified four types of lingual papillae: filiform, scale\u2010like filiform, fungiform, and circumvallate. A distinctive lingual torus was observed in all three species, dividing the tongue into proximal and distal regions, with large circumvallate papillae present in the posterior region. Histologically, the tongues of these species exhibited a stratified squamous keratinized or parakeratinized epithelium and its lamina propria of connective tissue in the mucosa and a muscular layer of visceral striated muscle. Taste buds were identified in fungiform and circumvallate papillae. Immunohistochemical analysis revealed positive detection of neurofilament and S\u2010100 proteins, indicating the presence of somatosensory innervation and suggesting its potential role in sensory perception. Neurofilament\u2010positive nerve bundles were localized within the muscular layer and lamina propria of mucosa, while S\u2010100 immunoreactivity was observed in nerve bundles near the taste buds and lamina propria of mucosa of filiform papillae. These findings suggest that, despite the lack of taste buds in filiform papillae, they may serve a sensory function, aiding in the manipulation and retention of prey during aerial capture. This study contributes to our understanding of the morphological and functional adaptations of the lingual papillae in molossid bats.\n                  <\/jats:p>","DOI":"10.1002\/ar.70229","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:05:43Z","timestamp":1779329143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lingual morphology of three urban molossid bats from South America: Filiform lingual papillae with somatosensory innervation"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9967-8393","authenticated-orcid":false,"given":"Florencia Evelyn","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Facultad de Medicina, Instituto de Qu\u00edmica B\u00e1sica y Aplicada del NEA (IQUIBA NEA\u2010UNNE\u2010CONICET), Laboratorio de Investigaciones Bioqu\u00edmicas (LIBIM) Universidad Nacional del Nordeste  Corrientes Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Olea","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad Nacional del Nordeste  Corrientes Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Sald\u00edvar\u2010Pintos","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad Nacional del Nordeste  Corrientes Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Flores\u2010Quintana","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias, C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad Nacional del Nordeste  Corrientes Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Vircotia","family":"Aguirre","sequence":"additional","affiliation":[{"name":"Facultad de Medicina, Instituto de Qu\u00edmica B\u00e1sica y Aplicada del NEA (IQUIBA NEA\u2010UNNE\u2010CONICET), Laboratorio de Investigaciones Bioqu\u00edmicas (LIBIM) Universidad Nacional del Nordeste  Corrientes Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Marcelo","family":"Lombardo","sequence":"additional","affiliation":[{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"},{"name":"Facultad de Ciencias Veterinarias, Instituto de Investigaci\u00f3n y Tecnolog\u00eda en Reproducci\u00f3n Animal (INITRA), C\u00e1tedra de Histolog\u00eda y Embriolog\u00eda Universidad de Buenos Aires  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"issue":"6","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"2192","DOI":"10.1002\/jemt.24076","article-title":"Lingual papillary system of the Egyptian mouse\u2010tailed bat (Rhinopoma hardwickii): New insights into its feeding strategies using scanning electron microscopy and immunohistochemical analysis of keratin","volume":"85","author":"Abumandour M. M.","year":"2022","journal-title":"Microscopy Research and Technique"},{"issue":"4","key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.4067\/S0717-95022014000400045","article-title":"Morphological comparison of the filiform papillae of New Zealand white rabbits (Oryctolagus cuniculus) and Egyptian fruit bats (Rousettus aegyptiacus) using scanning electron microscopy","volume":"32","author":"Abumandour M. M. A.","year":"2014","journal-title":"International Journal of Morphology"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.15560\/12.2.1873"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.31687\/saremNMS.19.0.12"},{"key":"e_1_2_10_6_1","first-page":"1","article-title":"Nueva gu\u00eda de los murci\u00e9lagos de Argentina","volume":"3","author":"Barquez R. M.","year":"2020","journal-title":"Publ Esp"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5962\/bhl.title.142628","volume-title":"The bats of Argentina","author":"Barquez R. M.","year":"1999"},{"issue":"2","key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"104","DOI":"10.30972\/vet.2321787","article-title":"Identificaci\u00f3n y caracterizaci\u00f3n de refugios de quir\u00f3pteros en la ciudad de Corrientes, Argentina","volume":"23","author":"Bastiani C. E.","year":"2012","journal-title":"Revista Veterinaria"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1590\/S0101-81752002000100016","article-title":"Diet, activity and reproduction of bat species (Mammalia, Chiroptera) in Central Amazonia, Brazil","volume":"19","author":"Bernard E.","year":"2002","journal-title":"Revista Brasileira de Zoologia"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1126\/science.1201366","article-title":"Economic importance of bats in agriculture","volume":"332","author":"Boyles J. G.","year":"2011","journal-title":"Science"},{"key":"e_1_2_10_11_1","first-page":"398","volume-title":"Bat ecology","author":"Dumont E. R.","year":"2003"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1111\/j.1095-8312.1988.tb00811.x","article-title":"Frugivorous and animalivorous bats (Microchiroptera): Dental and cranial adaptations","volume":"33","author":"Freeman P. W.","year":"1988","journal-title":"Biological Journal of the Linnean Society"},{"issue":"22","key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"4578","DOI":"10.1111\/j.1742-4658.2010.07887.x","article-title":"Natural and amyloid self\u2010assembly of S100 proteins: Structural basis of functional diversity","volume":"277","author":"Fritz G.","year":"2010","journal-title":"The FEBS Journal"},{"key":"e_1_2_10_14_1","first-page":"293","volume-title":"Biology of bats","author":"Gardner A. L.","year":"1977"},{"key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.brainres.2007.05.070","article-title":"Differential distribution of S100 protein and calretinin in mechanosensory and chemosensory cells of adult zebrafish (Danio rerio)","volume":"1162","author":"German\u00e0 A.","year":"2007","journal-title":"Brain Research"},{"issue":"6","key":"e_1_2_10_16_1","first-page":"41","article-title":"Histological study of tongue in Rousettus aegyptiacus","volume":"1","author":"Ghassemi F.","year":"2013","journal-title":"International Journal for Research in Applied and Natural Science"},{"key":"e_1_2_10_17_1","first-page":"41","article-title":"Lingual morphology and cladistic analysis of New World nectar\u2010feeding bats (Chiroptera: Phyllostomidae)","volume":"1","author":"Gimenez E. A.","year":"1996","journal-title":"Journal of Comparative Biology"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1590\/S0073-47212003000200014","article-title":"Comparative morphology of the tongue in free\u2010tailed bats (Chiroptera, Molossidae)","volume":"93","author":"Gregorin R.","year":"2003","journal-title":"Iheringia Serie Zoologia"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","DOI":"10.11606\/1807-0205\/2021.61.86","article-title":"Comparative morphology of tongue surface in Neotropical aerial insectivore bats (Mammalia: Chiroptera)","volume":"61","author":"Gregorin R.","year":"2021","journal-title":"Pap\u00e9is Avulsos de Zoologia"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/BF00328001","article-title":"Mouse adrenal chromaffin cells can transform to neuron\u2010like cholinergic phenotypes after being grafted into the brain","volume":"274","author":"Jousselin\u2010Hosaja M.","year":"1993","journal-title":"Cell and Tissue Research"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.biocon.2007.04.009","article-title":"Effects of wastewater treatment plant effluent on bat foraging ecology in an urban stream system","volume":"138","author":"Kalcounis\u2010Rueppell M. C.","year":"2007","journal-title":"Biological Conservation"},{"issue":"6","key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1097\/00024382-199912000-00012","article-title":"Histological and histochemical methods: Theory and practice","volume":"12","author":"Kiernan J. A.","year":"1999","journal-title":"Shock"},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.2011.06004.x","article-title":"Ecosystem services provided by bats","volume":"1223","author":"Kunz T. H.","year":"2011","journal-title":"Annals of the New York Academy of Sciences"},{"key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1093\/oso\/9780198549451.003.0008","article-title":"Maternal investment and post\u2010natal growth in bats","author":"Kunz T. H.","year":"1995","journal-title":"Ecology, Evolution and Behaviour of Bats"},{"issue":"1","key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1644\/1545-1542(2005)086<0067:DVOBFB>2.0.CO;2","article-title":"Dietary variation of Brazilian free\u2010tailed bats links to migratory populations of pest insects","volume":"86","author":"Lee Y. F.","year":"2005","journal-title":"Journal of Mammalogy"},{"issue":"1","key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.3161\/15081109ACC2019.21.1.004","article-title":"Not all Molossus are created equal: Genetic variation in the mastiff bat reveals diversity masked by conservative morphology","volume":"21","author":"Loureiro L. O.","year":"2019","journal-title":"Acta Chiropterologica"},{"issue":"2","key":"e_1_2_10_27_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.acthis.2020.151503","article-title":"Anatomical features of the tongue of two chiropterans endemic in the Egyptian fauna: The Egyptian fruit bat (Rousettus aegyptiacus) and insectivorous bat (Pipistrellus kuhlii)","volume":"122","author":"Massoud D.","year":"2020","journal-title":"Acta Histochemica"},{"issue":"1","key":"e_1_2_10_28_1","first-page":"3","article-title":"Qu\u00e9 tienen los murci\u00e9lagos, que unos los quieren destruir y otros los quieren salvar?","volume":"9","author":"Medell\u00edn R. A.","year":"2000","journal-title":"Revista Especies"},{"issue":"2","key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"112087","DOI":"10.1016\/j.celrep.2023.112087","article-title":"The cellular basis of mechanosensation in mammalian tongue","volume":"42","author":"Moayedi Y.","year":"2023","journal-title":"Cell Reports"},{"issue":"5","key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1016\/j.acthis.2018.05.010","article-title":"Anatomical, histological and immunohistochemical study of testicular development in Columba livia (Aves: Columbiformes)","volume":"120","author":"Olea G. B.","year":"2018","journal-title":"Acta Histochemica"},{"issue":"1","key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"64","DOI":"10.2307\/3626255","article-title":"The gross anatomy of the tongues and stomachs of eight New World bats","volume":"54","author":"Park H.","year":"1951","journal-title":"Transactions of the Kansas Academy of Science"},{"issue":"3","key":"e_1_2_10_32_1","first-page":"267","article-title":"Comparative morphology of the tongue of Miniopterus schreibersi fuliginosus and Pipistrellus savii","volume":"39","author":"Park J. W.","year":"2009","journal-title":"Applied Microscopy"},{"issue":"2","key":"e_1_2_10_33_1","first-page":"101","article-title":"Morphological study on the dorsal lingual papillae of Sorex caecutiens","volume":"39","author":"Park J. W.","year":"2009","journal-title":"Applied Microscopy"},{"issue":"7","key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/0003-9969(93)90125-6","article-title":"Morphological study by scanning electron microscopy of the lingual papillae in the common European bat (Pipistrellus pipistrellus)","volume":"38","author":"Pastor J. F.","year":"1993","journal-title":"Archives of Oral Biology"},{"issue":"1","key":"e_1_2_10_35_1","first-page":"37","article-title":"Morphological studies on the lingual papillae with special reference to circumvallate papillae of insectivorous bat Taphozous longimanus","volume":"1","author":"Ramteke A. V.","year":"2013","journal-title":"International Journal of Life Sciences"},{"issue":"5","key":"e_1_2_10_36_1","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1603\/033.046.0510","article-title":"Experimental assessment of the impacts of northern long\u2010eared bats on ovipositing Culex mosquitoes","volume":"46","author":"Reiskind M. H.","year":"2009","journal-title":"Journal of Medical Entomology"},{"issue":"11","key":"e_1_2_10_37_1","doi-asserted-by":"crossref","first-page":"2888","DOI":"10.1002\/ar.25277","article-title":"Comparative study of the gular gland of three species of Molossidae bats (Mammalia: Chiroptera) from South America","volume":"306","author":"Rodriguez F. E.","year":"2023","journal-title":"The Anatomical Record"},{"issue":"3","key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1093\/jmammal\/gyw078","article-title":"Guidelines of the American Society of Mammalogists for the use of wild mammals in research and education","volume":"97","author":"Sikes R. S.","year":"2016","journal-title":"Journal of Mammalogy"},{"key":"e_1_2_10_39_1","first-page":"493","volume-title":"Bat ecology","author":"Simmons N. B.","year":"2003"},{"issue":"12","key":"e_1_2_10_40_1","doi-asserted-by":"crossref","DOI":"10.3390\/cells11121899","article-title":"Alpha\u2010keratin, keratin\u2010associated proteins and transglutaminase 1 are present in the ortho\u2010 and parakeratinized epithelium of the avian tongue","volume":"11","author":"Skieresz\u2010Szewczyk K.","year":"2022","journal-title":"Cells"},{"issue":"2","key":"e_1_2_10_41_1","first-page":"633","article-title":"Comparative histological studies on the tongue of three species of Egyptian bats","volume":"10","author":"Taki\u2010El\u2010Deen F. M.","year":"2013","journal-title":"Life Science Journal"},{"issue":"1","key":"e_1_2_10_42_1","first-page":"82","article-title":"AVMA guidelines for the euthanasia of animals","volume":"1","author":"Underwood W.","year":"2013","journal-title":"Journal of the American Veterinary Medical Association"},{"key":"e_1_2_10_43_1","first-page":"315","volume-title":"Ecological and behavioral methods for the study of bats","author":"Wilkinson G. S.","year":"2009"},{"issue":"3","key":"e_1_2_10_44_1","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1644\/BWG-032","article-title":"Operational tongue length in phyllostomid nectar\u2010feeding bats","volume":"84","author":"Winter Y.","year":"2003","journal-title":"Journal of Mammalogy"}],"container-title":["The Anatomical Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.70229","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ar.70229","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.70229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T02:05:49Z","timestamp":1779329149000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.70229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":43,"alternative-id":["10.1002\/ar.70229"],"URL":"https:\/\/doi.org\/10.1002\/ar.70229","archive":["Portico"],"relation":{},"ISSN":["1932-8486","1932-8494"],"issn-type":[{"value":"1932-8486","type":"print"},{"value":"1932-8494","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]},"assertion":[{"value":"2025-12-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ar.70229"}}