{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T07:00:09Z","timestamp":1781161209445,"version":"3.54.1"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004916","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Amazonas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004916","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The claspers are the copulatory organs in male elasmobranchs, responsible for directing the semen into the female cloaca (C). However, the microscopic morphology of the claspers remains poorly understood. This study describes the morphology of the claspers and clasper glands (CGs) of cururu stingray (\n                    <jats:italic>Potamotrygon wallacei<\/jats:italic>\n                    ) at different maturational stages, including neonates, juveniles, and adult active males. These secondary sexual organs were analyzed anatomically and processed using standard histological and histochemical staining techniques. The claspers of\n                    <jats:italic>P. wallacei<\/jats:italic>\n                    have a tubular shape and a brown coloration with light spots, intensified in juveniles and active males. Claspers are flexible in neonates, show an increased rigidity in juveniles, and are fully rigid in adults. Gradual rigidity of the claspers results from mesenchyme (ME) replacement by cartilage followed by mineralization. The claspers are lined by an epidermis (EP) with mucous cells (MC) secreting neutral and acid carboxylate mucosubstances; spermatic groove (SG) with cells exhibiting neutral mucosubstances; vascularized connective tissue and musculature associated with nerve bundles. Such tissue characteristics can assist in penetration, protection, rotational movements, and opening of the claspers during copulation. The CG is recognizable in neonates and becomes progressively more structured through ontogeny, reaching full differentiation in adults. The CG is delimited by two external muscular tunics, with secretory units (SU) formed by a simple columnar epithelium, demonstrating a holocrine secretion with neutral and acid carboxylate mucosubstances, more intense in adults. This secretion can assist in sperm capacitation, nutrition, and motility. These results confirm that secondary sexual organs undergo morphophysiological changes during sexual maturation in\n                    <jats:italic>P. wallacei<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/ar.70244","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:40:14Z","timestamp":1781160014000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Developmental morphology of claspers and clasper\u2010glands during maturation in\n                    <i>Potamotrygon wallacei<\/i>\n                    (Chondrichthyes: Potamotrygoninae)"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9997-7243","authenticated-orcid":false,"given":"Matheus Samuel Cunha","family":"Braga","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biologia de \u00c1gua Doce e Pesca Interior (PPG\u2010 BADPI) Instituto Nacional de Pesquisas da Amaz\u00f4nia (INPA)  Manaus AM Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2894-7277","authenticated-orcid":false,"given":"Grazyelle Sebrenski","family":"da Silva","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Biologia de \u00c1gua Doce e Pesca Interior (PPG\u2010 BADPI) Instituto Nacional de Pesquisas da Amaz\u00f4nia (INPA)  Manaus AM Brazil"},{"name":"Departamento de Morfologia Instituto de Ci\u00eancias Biol\u00f3gicas (ICB), Universidade Federal do Amazonas (UFAM)  AM Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8408-8761","authenticated-orcid":false,"given":"Maria In\u00eas Braga","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Departamento de Morfologia Instituto de Ci\u00eancias Biol\u00f3gicas (ICB), Universidade Federal do Amazonas (UFAM)  AM Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5141-7816","authenticated-orcid":false,"given":"Jaydione Luiz","family":"Marcon","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Fisiol\u00f3gicas Instituto de Ci\u00eancias Biol\u00f3gicas (ICB), Universidade Federal do Amazonas (UFAM)  AM Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0306-8206","authenticated-orcid":false,"given":"Ruben Dario Morales","family":"Gamba","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Peces Neotropicales Fundaci\u00f3n para la Investigaci\u00f3n y Desarrolo Sostenible (FUNINDES\u2010Colombia)  Cali Valle del Cauca Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.20647"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.1850"},{"key":"e_1_2_11_4_1","unstructured":"Ara\u00fajo M. L. G.(1998).Biologia reprodutiva e pesca dePotamotrygonsp. C (Chondrichthyes Potamotrygonidae) no m\u00e9dio rio Negro Amazonas. Master Thesis Instituto Nacional de Pesquisas da Amaz\u00f4nia Manaus Amazonas."},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.762042"},{"key":"e_1_2_11_6_1","first-page":"173","volume-title":"Bancroft's theory and practice of histological techniques","author":"Bancroft J. D.","year":"2012"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0319354"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10641-024-01551-0"},{"key":"e_1_2_11_9_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1201\/9780203491317.ch10","volume-title":"Biology of sharks and their relatives","author":"Carrier J. 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