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In mammals, notable adaptations include evolving hypsodonty for grazing on grasses and, for bats, evolving echolocation and wing acquisition. Recent studies have highlighted the overlooked morphological diversity of the bat larynx, a key organ for echolocation capabilities. Tracheal chambers, found on the trachea's first rings, are enigmatic components within the laryngeal complex whose origins and functions remain incompletely understood. We hypothesized that these structures demonstrate evolutionary convergence and represent a key innovation responsible for the diversification of laryngeal echolocation behaviors. By analyzing 51 bat species, their laryngeal cartilages, and tracheal chambers, we explored relationships between body mass, sound frequencies, and chamber volumes. We hypothesized that tracheal chambers likely facilitated laryngeal echolocation capabilities in bats. Ancestral state reconstructions were conducted to understand the evolution of tracheal chambers and echolocation behaviors in bats. We conclude that tracheal chambers might support higher frequency sound production and could have been pivotal for high\u2010duty cycle echolocation specialization during the development of nasally emitted calls, which allowed bats to thrive in diverse environments. We suggest that tracheal chambers are specialized phenotypic adaptations that might have helped in diversifying laryngeal echolocation behaviors and the evolutionary success of bats.<\/jats:p>","DOI":"10.1002\/ar.70266","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T16:25:16Z","timestamp":1783355116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tracheal chambers as a key innovation for high\u2010frequency emission in bat echolocation"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1367-0778","authenticated-orcid":false,"given":"Nicolas L. 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B.","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Archaeology and Anthropology, College of Arts and Social Sciences The Australian National University  Acton Australian Capital Territory Australia"},{"name":"School of Biological, Earth and Environmental Sciences University of New South Wales  Kensington New South Wales Australia"},{"name":"ARC Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing, Research School of Physics The Australian National University  Acton Australian Capital Territory Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0950-9351","authenticated-orcid":false,"given":"Khizar","family":"Hayat","sequence":"additional","affiliation":[{"name":"Jockey Club College of Veterinary Medicine and Life Sciences City University of Hong Kong  Hong Kong SAR China"},{"name":"Department of Clinical Sciences, College of Veterinary Medicine Cornell University  Ithaca New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8021-8127","authenticated-orcid":false,"given":"Michael","family":"Doube","sequence":"additional","affiliation":[{"name":"School of Ecology Sun Yat\u2010sen University  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5915-865X","authenticated-orcid":false,"given":"Vuong Tan","family":"Tu","sequence":"additional","affiliation":[{"name":"Institute of Biology, Vietnam Academy of Science and Technology  Hanoi Vietnam"},{"name":"Graduate University of Science and Technology Vietnam Academy of Science and Technology  Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3146-1849","authenticated-orcid":false,"given":"Thongchai","family":"Ngamprasertwong","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6695-1015","authenticated-orcid":false,"given":"Thanakul","family":"Wannaprasert","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4087-7742","authenticated-orcid":false,"given":"Daisuke","family":"Koyabu","sequence":"additional","affiliation":[{"name":"School of Ecology Sun Yat\u2010sen University  Shenzhen China"},{"name":"School of Archaeology and Anthropology, College of Arts and Social Sciences The Australian National University  Acton Australian Capital Territory Australia"},{"name":"Research and Development Center for Precision Medicine University of Tsukuba  Tsukuba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Appel G. 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