{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T07:10:54Z","timestamp":1783926654746,"version":"3.55.0"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201006682\u201024"],"award-info":[{"award-number":["APQ\u201006682\u201024"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Anatomical Record"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The organization and function of reptilian excurrent ducts remain poorly characterized, particularly in Neotropical snakes. We provide the first integrative morphological, histochemical, immunohistochemical, and ultrastructural analysis of the proximal post\u2010testicular ducts of\n                    <jats:italic>Crotalus durissus<\/jats:italic>\n                    across reproductive stages. Using light, transmission electron, and confocal laser microscopy, we examined the rete testis, ductuli efferentes, ductuli epididymides, and ductus epididymis of adult males. The proximal post\u2010testicular ducts are contained within the epididymal sheath alongside the adrenal gland. No anatomical regionalization was identified along the epididymis. The ductuli efferentes exhibited cuboidal epithelium with stereocilia\u2010like projections, while the ductuli epididymides displayed simple columnar epithelium with abundant periodic acid\u2010Schiff (PAS) and Bromophenol Blue (BB) positive secretory granules. The ductus epididymis consisted of pseudostratified epithelium with principal, basal, and clear cells. Confocal analyses revealed wheat germ agglutinin (WGA) in principal cells of the ductus epididymis and Concanavalin A (ConA) positive secretory granules in ductuli epididymides. Aquaporin\u20109 immunolabeling was detected in the epithelium of the ductus epididymis and ductuli efferentes, suggesting a role in luminal fluid regulation. Ultrastructural observations of the ductus epididymis revealed extensive rough endoplasmic reticulum, Golgi complexes, microvilli, and phagolysosomes in principal cells, supporting secretory, absorptive, and phagocytic functions. Seasonal variation was evident, with snakes in testicular activity exhibiting hypertrophy of all components analyzed. Collectively, our findings indicate that these ducts of\n                    <jats:italic>C. durissus<\/jats:italic>\n                    are functionally specialized for secretion and absorption, rather than sperm storage, thereby contributing to the establishment and regulation of a luminal microenvironment essential for sperm maturation in this seasonally reproducing viperid.\n                  <\/jats:p>","DOI":"10.1002\/ar.70287","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T06:50:03Z","timestamp":1783925403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Morphophysiological dynamics of the proximal post\u2010testicular ducts in\n                    <i>Crotalus durissus<\/i>\n                    Linnaeus, 1758 (Squamata: Viperidae)"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2889-6051","authenticated-orcid":false,"given":"Fl\u00e1via Cappuccio","family":"de Resende","sequence":"first","affiliation":[{"name":"Herpetology and Animal Histology Laboratory Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6922-5677","authenticated-orcid":false,"given":"Gustavo Caldeira","family":"Cotta","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Morphology Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara d' \u00c1vila Fonseca Paiva","family":"de Paula Freitas","sequence":"additional","affiliation":[{"name":"Herpetology and Animal Histology Laboratory Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Herpetology and Animal Histology Laboratory Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helo\u00edsa Helena Marques","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Herpetology and Animal Histology Laboratory Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vict\u00f3ria Gomes Carvalho","family":"Silva","sequence":"additional","affiliation":[{"name":"Herpetology and Animal Histology Laboratory Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia","family":"de F\u00e1tima Moreira Silva","sequence":"additional","affiliation":[{"name":"Herpetology and Animal Histology Laboratory Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Maria","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology Ezequiel Dias Foundation  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samyra Maria","family":"dos Santos Nassif Lacerda","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Morphology Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilherme Mattos Jardim","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology, Department of Morphology Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,12]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"36","article-title":"Image processing with ImageJ","volume":"11","author":"Abr\u00e0moff M. 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