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However, systematic quantitative data on axon\u2010profile counts and marker\u2010defined axonal composition at intermediate anatomical levels, particularly trunks and divisions, remain limited. Moreover, quantitative data on fascicle numbers across different anatomical levels of the brachial plexus remain scarce. Here, we analyzed brachial plexus specimens from 10 human donors using NF200\/ChAT dual immunofluorescence staining to assess axon\u2010profile counts and fascicular organization across five sampled anatomical levels: roots, trunks, divisions, cords, and terminal branches. Within the sampled structures, NF200\u2010positive axon\u2010profile counts were numerically higher at proximal levels and lower at more distal sampled levels, whereas ChAT\/NF200 double\u2010positive profile counts showed less variation, with their proportional contribution increasing from 12.61% to 20.84%. Fascicle numbers were numerically highest at the trunk and cord levels. On representative qualitative inspection, no stable intrafascicular spatial pattern of ChAT\/NF200 double\u2010positive profiles was apparent. This study provides marker\u2010defined quantitative anatomical reference data on NF200\u2010positive axon\u2010profile counts, ChAT\/NF200\u2010defined axonal composition, and fascicle numbers across five sampled anatomical levels of the human brachial plexus, including the under\u2010quantified trunks and divisions. These data may serve as a foundation for future studies of brachial plexus anatomy, nerve repair planning, and neural interface design.<\/jats:p>","DOI":"10.1002\/ar.70321","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T01:55:26Z","timestamp":1788832526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative mapping of\n                    <scp>NF200<\/scp>\n                    \/\n                    <scp>ChAT<\/scp>\n                    \u2010defined axonal profiles across the anatomical hierarchy of the human brachial plexus"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2161-6397","authenticated-orcid":false,"given":"Yunuo","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Hand Surgery Huashan Hospital, Fudan University  Shanghai China"},{"name":"Department of Hand and Upper Extremity Surgery Jing'an District Central Hospital, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4837-875X","authenticated-orcid":false,"given":"Zhen","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Hand Surgery Huashan Hospital, Fudan University  Shanghai China"},{"name":"Department of Hand and Upper Extremity Surgery Jing'an District Central Hospital, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huitao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hand Surgery Huashan Hospital, Fudan University  Shanghai China"},{"name":"Department of Hand and Upper Extremity Surgery Jing'an District Central Hospital, Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hand and Upper Extremity Surgery Jing'an District 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