{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:30:07Z","timestamp":1791300607023,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,5,28]],"date-time":"2003-05-28T00:00:00Z","timestamp":1054080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fondo de Investigaciones Sanitarias (Instituto de Salud Carlos III)","award":["FIS 01\/0008-01"],"award-info":[{"award-number":["FIS 01\/0008-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We studied the morphology of cortical microvessels in the brains of 10 patients who had died after receiving a traumatic head injury (THI). Scanning electron microscopy (SEM) of vascular corrosion casts, confocal microscopy of histological sections after immunocytochemistry, and detection of apoptosis by terminal dUTP nick end labeling (TUNEL) were used. Microvascular casts showed an angioarchitectonic distribution that was defined as normal according to results obtained in a previous, nontraumatic series of subjects. However, when we compared them with previous works, the cast surface of some of the microvessels showed three types of morphological alterations: longitudinal folds, sunken surfaces with craters, and a significant flattening with reduction of lumen. The vessels that were primarily affected were the arterioles and capillaries of the middle and deep cortical vascular zones. Immunostaining with the monoclonal antibody MAS\u2010336 against endothelial cells also showed the presence of longitudinal folds with a thinning of the vascular lumen, cytoplasmic round bodies, and a thickening of the endothelial cell membrane. The TUNEL technique revealed a positive staining of some endothelial cells.<\/jats:p><jats:p>The structural alterations we observed indicate that microvessels undergo endothelial cell damage after THI. We suggest that this kind of lesion and the secondary functional injury to the blood\u2013brain barrier (BBB) could play an important role in the development of the secondary lesions that these patients show in the subacute phase. Anat Rec Part A 273A:583\u2013593, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.a.10069","type":"journal-article","created":{"date-parts":[[2003,6,10]],"date-time":"2003-06-10T06:09:30Z","timestamp":1055225370000},"page":"583-593","source":"Crossref","is-referenced-by-count":106,"title":["Morphological features in human cortical brain microvessels after head injury: A three\u2010dimensional and immunocytochemical study"],"prefix":"10.1002","volume":"273A","author":[{"given":"Alfonso","family":"Rodr\u00edguez\u2010Baeza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Reina\u2010de la Torre","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Poca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merc\u00e8","family":"Mart\u00ed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel","family":"Garnacho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,5,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1179\/016164101101198514"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000267986"},{"key":"e_1_2_6_4_1","first-page":"384","article-title":"Cerebral blood flow in severe clinical head injury","volume":"3","author":"Bouma GJ","year":"1995","journal-title":"New Horiz"},{"key":"e_1_2_6_5_1","first-page":"145","article-title":"Electron microscopic analysis of cortical biopsies in patients with traumatic brain injuries and dysfunction of neurobehavioral system","volume":"30","author":"Castej\u00f3n OJ","year":"1998","journal-title":"J Submicrosc Cytol Pathol"},{"key":"e_1_2_6_6_1","first-page":"95","article-title":"Structures imitating myocites and pericytes in corrosion casts of terminal blood vessels. 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