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ER\u03b1 and ER\u03b2 showed cell\u2010type\u2010 and species\u2010specific distributions, respectively. In contrast, AR was localized in the epithelial and stroma cells of all tissues examined in this study, in both species. In mice, the epithelial cells of the ductuli efferentes showed a strong ER\u03b1\u2010immunoreaction, and those of the caput epididymis, coagulating glands, and prostate also exhibited a positive reaction. Stroma cells, except in the ductuli efferentes, showed a positive ER\u03b1\u2010immunostaining. In rats, ER\u03b1 was detected in very few cell types: the epithelial cells of the ductuli efferentes showed a strong reaction, and the stroma cells of the ampullary and urethral glands exhibited a weak reaction. ER\u03b2 was localized in the epithelial cells of the prostate in mice, while the reaction was faint or negative in both the epithelial and stroma cells of other tissues. In rats, the ER\u03b2\u2010immunoreaction was strongest in the epithelial cells of the ventral prostate. The epithelial cells of the corpus and cauda epididymis, ductus deferens, and urethral glands, and the stroma cells of the urethral glands were also positively ER\u03b2\u2010immunostained. Almost the same AR distribution pattern was observed in both species. In particular, strong AR\u2010immunostaining was present in the epithelial cells of the caput and corpus epididymis, seminal vesicle, and ventral prostate. These results indicate that species and tissues differences should be taken into careful consideration in assessing the physiological and pharmacological effects of sex steroids (particularly estrogens) on the reproductive tissues of male rodents. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.a.20061","type":"journal-article","created":{"date-parts":[[2004,7,5]],"date-time":"2004-07-05T03:02:10Z","timestamp":1088996530000},"page":"768-778","source":"Crossref","is-referenced-by-count":70,"title":["Localization of estrogen and androgen receptors in male reproductive tissues of mice and rats"],"prefix":"10.1002","volume":"279A","author":[{"given":"Shuji","family":"Yamashita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"874","DOI":"10.1210\/endo.142.2.7978","article-title":"Age\u2010, 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