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Rec."],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of triiodo\u2010L\u2010thyronine (T<jats:sub>3<\/jats:sub>) and propylthiouracil (PTU) on the initiation of epidermal growth factor (EGF) expression in the sublingual glands (SLGs) of postnatal mice was investigated by indirect enzyme\u2010labeled and immunogold antibody methods for light and electron microscopy, respectively. In normal males, EGF immunoreactivity first appeared in a few scattered granular cells of striated ducts (SDs) at 5 weeks of age, and the immunoreactive cells had increased in number at 6 weeks of age. No EGF expression was observed in the glands of females at any ages examined. When T<jats:sub>3<\/jats:sub> (1 mg\/kg body weight) was given to males every other day for 2 weeks before examination, EGF expression began earlier; the immunoreactive granular cells were first detected at 4 weeks of age, and at later ages they were markedly increased in number compared to those of normal males. Moreover, T<jats:sub>3<\/jats:sub> was capable of inducing EGF in the female glands. After T<jats:sub>3<\/jats:sub> was administered to females in the same manner as in males, a few immunoreactive cells were first detected at 5 weeks of age, and increased numbers were detected at later ages. By contrast, when PTU (1 mg\/kg body weight) was given to male mice every other day for 2 weeks before examination, the EGF\u2010immunoreactive cells were markedly decreased in number compared to those of normal males of the same age. Electron microscopy revealed that many SD cells contained secretory granules, and that these cells constituted the granular striated tubule (GST) in a portion of SDs, but they were undetectable by light microscopy, because their secretory granules were minimal in size and few in number. Gold\u2010labeling of EGF was confined to the secretory granules of scattered granular cells, whose secretory granules were far larger in size and more abundant than those of the GST cells. These results suggest that thyroid hormone is essential to differentiation of the cellular phenotype of GST precursor cells into typical granular cells (detectable by light microscopy) that express EGF in the mouse SLG, showing a close resemblance to the submandibular granular convoluted tubule cells. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.a.20197","type":"journal-article","created":{"date-parts":[[2005,5,11]],"date-time":"2005-05-11T16:17:49Z","timestamp":1115828269000},"page":"585-593","source":"Crossref","is-referenced-by-count":8,"title":["Role of thyroid hormone in the initiation of EGF (epidermal growth factor) expression in the sublingual gland of the postnatal mouse"],"prefix":"10.1002","volume":"284A","author":[{"given":"Kazumasa","family":"Yoshida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeo","family":"Aiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoru","family":"Uchida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shingo","family":"Kurabuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(80)90183-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01002465"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/28.8.7003007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00218202"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60101-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-96-6-1571"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001510102"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001530307"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001590206"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-109-3-924"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092050207"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070270102"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1093\/oxfordjournals.jbchem.a133461","article-title":"Induction of epidermal growth factor by tri\u2010iod\u2010thyronine in the submandibular glands of mice with testicular feminization","volume":"90","author":"Hosoi K","year":"1981","journal-title":"J Biochem"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/41.5.8468451"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001210309"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001490108"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1003506302065"},{"key":"e_1_2_6_19_1","unstructured":"KurabuchiS HosoiK GresikEW.2000.Sexually dimorphic distribution of a true tissue kallikrein mK1 in the striated ducts of the mouse sublingual gland. 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