{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T04:37:11Z","timestamp":1789965431962,"version":"4.0.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anat. Rec."],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The majority of complex congenital heart defects occur in individuals who are afflicted by laterality disease. We hypothesize that the prevalence of valvuloseptal defects in this population is due to defective left\u2010right patterning of the embryonic atrioventricular (AV) canal cushions, which are the progenitor tissue for valve and septal structures in the mature heart. Using embryos of the frog <jats:italic>Xenopus laevis<\/jats:italic>, this hypothesis was tested by performing left\u2010right lineage analysis of myocytes and cushion mesenchyme cells of the superior and inferior cushion regions of the AV canal. Lineage analyses were conducted in both wild\u2010type and laterality mutant embryos experimentally induced by misexpression of ALK4, a type I TGF\u2010\u03b2 receptor previously shown to modulate left\u2010right axis determination in Xenopus. We find that abnormalities in overall amount and left\u2010right cell lineage composition are present in a majority of ALK4\u2010induced laterality mutant embryos and that much variation in the nature of these abnormalities exists in embryos that exhibit the same overall body situs. We propose that these two parameters of cushion tissue formation\u2014amount and left\u2010right lineage origin\u2014are important for normal processes of valvuloseptal morphogenesis and that defective allocation of cells in the AV canal might be causatively linked to the high incidence of valvuloseptal defects associated with laterality disease. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/ar.a.20269","type":"journal-article","created":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T16:23:34Z","timestamp":1132244614000},"page":"1176-1182","source":"Crossref","is-referenced-by-count":14,"title":["Left\u2010right lineage analysis of AV cushion tissue in normal and laterality defective Xenopus hearts"],"prefix":"10.1002","volume":"287A","author":[{"given":"Ann F.","family":"Ramsdell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayne M.","family":"Bernanke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Trusk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/1058-9813(96)00171-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.4.827"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2003.12.035"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.77.1.1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.11.969"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00427-003-0337-5"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90189-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2004.08.006"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00188218"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(19990601)255:2<212::AID-AR11>3.0.CO;2-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-1788-6_2"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9559"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1221"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199705)248:1<84::AID-AR10>3.0.CO;2-1"},{"key":"e_1_2_6_16_1","volume-title":"Normal table of Xenopus laevis (Daudin)","author":"Nieuwkoop PD","year":"1967"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0706"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"5195","DOI":"10.1242\/dev.126.23.5195","article-title":"Cardiac looping and the left\u2010right axis: antagonism of left\u2010sided Vg1 activity by a right\u2010sided ALK2\u2010dependent BMP pathway","volume":"126","author":"Ramsdell AF","year":"1999","journal-title":"Development"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.07.038"},{"key":"e_1_2_6_20_1","volume-title":"Early development of Xenopus laevis: a laboratory manual","author":"Sive HL","year":"2000"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199607)206:3<301::AID-AJA7>3.0.CO;2-M"}],"container-title":["The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Far.a.20269","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ar.a.20269","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T17:29:27Z","timestamp":1759944567000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/ar.a.20269"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11,17]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,12]]}},"alternative-id":["10.1002\/ar.a.20269"],"URL":"https:\/\/doi.org\/10.1002\/ar.a.20269","archive":["Portico"],"relation":{},"ISSN":["1552-4884","1552-4892"],"issn-type":[{"value":"1552-4884","type":"print"},{"value":"1552-4892","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11,17]]}}}