{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T17:19:38Z","timestamp":1782235178472,"version":"3.54.5"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,6,11]],"date-time":"2003-06-11T00:00:00Z","timestamp":1055289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have isolated and characterised a <jats:italic>Triatoma infestans<\/jats:italic> cDNA encoding a lysozyme. A 174\u2010bp fragment was amplified by PCR using degenerate oligodeoxyribonucleotide primers derived from the known amino acid sequences of lysozyme from other insects. This PCR fragment was used to screen a cDNA gut library of <jats:italic>T. infestans<\/jats:italic>. A clone containing the 3\u2032\u2010end of the lysozyme cDNA (219 bp) was isolated and sequenced. RACE was used to amplify the 5\u2032\u2010end of the lysozyme cDNA. After sequencing the complete lysozyme cDNA, the deduced 417 amino acid sequence showed high identity (40\u201350%) with other chicken\u2010type lysozymes. The amino acid residues responsible for the catalytic activity and the binding of the substrate were essentially conserved. The expression pattern of the lysozyme gene in bugs at different molting and feeding states showed that this gene was upregulated in the digestive tract directly after the molt and after feeding. Additionally, this lysozyme gene was expressed differently in the different regions of the digestive tract, strongly in the cardia and stomach, the anterior regions of the midgut, and only traces of lysozyme mRNA could be detected in the small intestine, the posterior region of the midgut. Arch. Insect Biochem. Physiol. 53:134\u2013145, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/arch.10090","type":"journal-article","created":{"date-parts":[[2003,6,12]],"date-time":"2003-06-12T10:10:35Z","timestamp":1055412635000},"page":"134-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Isolation and characterization of a cDNA encoding for a lysozyme from the gut of the reduviid bug <i>Triatoma infestans<\/i>"],"prefix":"10.1002","volume":"53","author":[{"given":"Astrid H.","family":"Kollien","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Fechner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Waniek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter A.","family":"Schaub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,6,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_1_3_1","first-page":"19","article-title":"Secretion of colonic isozyme of lysozyme in association with cecrotrophy of rabbits","volume":"247","author":"C\u00e1mara VM","year":"1984","journal-title":"Am J Phys"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)67888-3"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(94)90033-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.7.1911"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00391008"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"11607","DOI":"10.1016\/S0021-9258(18)90907-5","article-title":"Stomach lysozymes of ruminants. 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