{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T15:55:33Z","timestamp":1770738933785,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,10,23]],"date-time":"2008-10-23T00:00:00Z","timestamp":1224720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In the present study, we used \u03b3\u2010ray to irradiate the female parasitoids to make wasp eggs infertile, resulting in pseudoparasitization, which allowed the analysis of maternal secretions such as polydnaviruses (PDVs) and venom in the absence of larval secretions or teratocytes by the growing parasitoids. We then investigated the spermatogenesis and components of testicular proteins of male <jats:italic>Plutella xylostella<\/jats:italic> larvae pseudoparasitized by two endoparasitoids (<jats:italic>Cotesia vestalis<\/jats:italic> and <jats:italic>Diadegma semiclausum<\/jats:italic>). The results showed that pseudoparasitism by the two endoparasitoids at the early third instar host larvae both induced smaller testes in size than those of nonparasitized host larvae. Both of them caused parasitic castration, and the degree of castration is almost as severe as in naturally parasitized hosts. This suggested that PDVs and venom played a major role in the degeneration of host testes. There are significant differences in the degree of castration induced by the two endoparasitoids, with respect to testicular growth, testicular protein concentrations, and histological changes of germ cells. <jats:italic>Cotesia vestalis<\/jats:italic> bracovirus always has a significantly stronger effect on host testicular growth and development than <jats:italic>D. semiclausum<\/jats:italic> ichnovirus. SDS\u2010PAGE analysis indicated that synthesis of P 65 and P 67 proteins were clearly inhibited in testes of hosts that were pseudoparasitized by <jats:italic>C. vestalis<\/jats:italic> while reduction in synthesis of other proteins was not evident. Arch. Insect Biochem. Physiol. 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/arch.20279","type":"journal-article","created":{"date-parts":[[2008,10,23]],"date-time":"2008-10-23T13:14:18Z","timestamp":1224767658000},"page":"30-43","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Parasitic castration of <i>Plutella xylostella<\/i> larvae induced by polydnaviruses and venom of <i>Cotesia vestalis<\/i> and <i>Diadegma semiclausum<\/i>"],"prefix":"10.1002","volume":"70","author":[{"given":"Su\u2010fen","family":"Bai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010zhang","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010xin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,10,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2653","DOI":"10.1099\/0022-1317-77-10-2653","article-title":"Host haemocyte inactivation by an insect parasitoid: transient expression of a polydnavirus gene","volume":"77","author":"Asgari S","year":"1996","journal-title":"J Gen 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