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The rank order of potency of the agonists was clonidine&gt;naphazoline&gt;tolazoline in Ca<jats:sup>2+<\/jats:sup>mobilization assays, and that of the antagonists was chlorpromazine&gt;yohimbine. These findings suggest that the<jats:italic>B. mori<\/jats:italic>OAR is more closely related to the class\u20101 OAR in the intact tissue than to the other classes.<jats:italic>N<\/jats:italic>\u2032\u2010(4\u2010Chloro\u2010<jats:italic>o<\/jats:italic>\u2010tolyl)\u2010<jats:italic>N<\/jats:italic>\u2010methylformamidine (DMCDM) and 2\u2010(2,6\u2010diethylphenylimino)imidazolidine (NC\u20105) elevated the intracellular calcium concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) with EC<jats:sub>50<\/jats:sub>s of 92.8\u2009\u00b5M and 15.2\u2009nM, respectively. DMCDM and NC\u20105 led to increases in intracellular cAMP concentration ([cAMP]<jats:sub>i<\/jats:sub>) with EC<jats:sub>50<\/jats:sub>s of 234\u2009nM and 125\u2009nM, respectively. The difference in DMCDM potencies between the cAMP and Ca<jats:sup>2+<\/jats:sup>assays might be due to \u201cfunctional selectivity.\u201d The Ca<jats:sup>2+<\/jats:sup>and cAMP assay results for DMCDM suggest that the elevation of [cAMP]<jats:sub>i<\/jats:sub>, but not that of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>, might account for the insecticidal effect of formamidine insecticides. \u00a9 2009 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/arch.20341","type":"journal-article","created":{"date-parts":[[2009,11,13]],"date-time":"2009-11-13T16:51:13Z","timestamp":1258131073000},"page":"74-86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Pharmacological characterization of a<i>Bombyx mori<\/i>\u03b1\u2010adrenergic\u2010like octopamine receptor stably expressed in a mammalian cell line"],"prefix":"10.1002","volume":"73","author":[{"given":"Jia","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Hamasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihisa","family":"Ozoe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,11,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03034.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf60203a011"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2004.02.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.1055"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151105198"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.60.6.1181"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-0456(01)00255-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.20076"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1981.sp013853"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1242\/jeb.129.1.239","article-title":"Phenyliminoimidazolidine derivatives activate both octopamine1 and octopamine2 receptor subtypes in locust skeletal muscle","volume":"129","author":"Evans PD","year":"1987","journal-title":"J Exp Biol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/287060a0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10158-005-0001-z"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00998270"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2007.06.005"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.51.2.293"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1327.052"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.01876.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-10-03650.1998"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.761457"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.208.4439.74"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi602593t"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.03.135"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.548"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tetasy.2004.10.027"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03251.x"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-3057(94)90352-2"},{"key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1016\/S0026-895X(25)14158-8","article-title":"Phenyliminoimidazolidines. 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