{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T22:19:49Z","timestamp":1792189189877,"version":"4.4.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,1,19]],"date-time":"2011-01-19T00:00:00Z","timestamp":1295395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this project, the uptake mechanisms and localization of two lectins from <jats:italic>Sambucus nigra<\/jats:italic>, further referred to as <jats:italic>S. nigra<\/jats:italic> agglutinin (SNA)\u2010I and SNA\u2010II, into insect midgut CF\u2010203 cells were studied. SNA\u2010I is a chimeric lectin belonging to the class of ribosome\u2010inactivating proteins, whereas SNA\u2010II is a hololectin devoid of enzymatic activity. Internalization of the fluorescein isothiocyanate\u2010labeled lectin was investigated using confocal microscopy. Both lectins were internalized into the cytoplasm of CF\u2010203 cells at similar rates. Preexposure of the insect midgut cells to specific inhibitors of clathrin\u2010 and caveolae\u2010mediated endocytosis resulted in an inhibition of lectin uptake in CF\u2010203 cells and caspase\u2010induced cytotoxicity caused by SNA\u2010I and SNA\u2010II, confirming the involvement of both endocytosis pathways. Further studies demonstrated that the uptake mechanism(s) for both lectins required phosphoinositide 3\u2010kinases, but did not depend on the actin cytoskeleton. Since the hololectin SNA\u2010II apparently uses a similar endocytosis pathway as the chimerolectin SNA\u2010I, it can be concluded that the endocytosis process mainly relies on the carbohydrate\u2010binding activity of the lectins under investigation. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/arch.20405","type":"journal-article","created":{"date-parts":[[2011,1,19]],"date-time":"2011-01-19T21:18:32Z","timestamp":1295471912000},"page":"211-222","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Internalization of <i>Sambucus nigra<\/i> agglutinins I and II in insect midgut CF\u2010203 cells"],"prefix":"10.1002","volume":"76","author":[{"given":"Shahnaz","family":"Shahidi\u2010Noghabi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Els J. M.","family":"Van Damme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Winnok H.","family":"De Vos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Smagghe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,1,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2960297"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(93)90002-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(04)00286-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.7972"},{"key":"e_1_2_6_6_1","first-page":"241","article-title":"The essential role of phosphoinositide 3\u2010kinases (PI3Ks) in regulating pro\u2010inflammatory responses and the progression of cancer","volume":"2","author":"Chen K","year":"2005","journal-title":"Cell Mol Immunol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.20048"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"18607","DOI":"10.1016\/S0021-9258(17)32353-0","article-title":"Selective regulation of apical endocytosis in polarized Madin\u2010Darby Canine Kidney cells by mastoparan and cAMP","volume":"269","author":"Eker P","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_6_9_1","first-page":"113","article-title":"Ribosome\u2010inactivating proteins and lectins from Sambucus","volume":"3","author":"Ferreras JM","year":"2000","journal-title":"Curr Topics Phytochem"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.12.4205"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085450"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.03.019"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(96)80146-2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.09.009"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(90)90576-K"},{"key":"e_1_2_6_16_1","first-page":"229","article-title":"Effect of repeated desipramine and fluoxetine administration on post\u2010adjuvant arthritis","volume":"52","author":"Kubera M","year":"2000","journal-title":"Polish J Pharmacol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2007.04.017"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"3449","DOI":"10.1242\/jcs.115.17.3449","article-title":"Selective regulation of the Rab9\u2010independent transport of ricin to the Golgi apparatus by calcium","volume":"17","author":"Lauvrak SU","year":"2002","journal-title":"J Cell Sci"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(99)00062-9"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-004-0652-6"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb787"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.52.1.785"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/35074539"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0751rev"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01681"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39337-8"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1996.76.4.949"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.22.5943"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-001-0346-2"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00294"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2008.09.012"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-008-9215-2"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2010.03.012"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M009414200"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1242\/jcs.109.12.2927","article-title":"The effects of cytochalasin D and phorbol myristate acetate on the apical endocytosis of ricin in polarised Caco\u20102 cells","volume":"109","author":"Shurety W","year":"1996","journal-title":"J Cell Sci"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2748"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6078-7"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2004.08.041"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.20.3737"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.00128.x"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0505k.x"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-008-9123-z"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-001-0371-1"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2006.04.009"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.6.2731"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47429-2"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Farch.20405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.20405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T12:21:05Z","timestamp":1700482865000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.20405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,19]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1002\/arch.20405"],"URL":"https:\/\/doi.org\/10.1002\/arch.20405","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,19]]},"assertion":[{"value":"2010-10-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-01-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}