{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:14:14Z","timestamp":1758399254934},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T00:00:00Z","timestamp":1316131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We are interested in identifying targets that may be used to develop new control products for the common housefly, <jats:italic>Musca domestica<\/jats:italic>, a vector of disease for many vertebrates. One such target, ornithine decarboxylase (ODC), is an embryonic enzyme involved in the regulation of polyamines and is a critical enzyme during <jats:italic>M. domestica<\/jats:italic> development. In this study, the cDNA for <jats:italic>ODC<\/jats:italic> from <jats:italic>M. domestica<\/jats:italic> was cloned, sequenced, and characterized. The full\u2010length cDNA was 1,337\u2010bp, consistent with a single band of approximately 1.35\u2009kb obtained by northern analysis. The open\u2010reading frame contains 1,191\u2009bp, yielding a deduced polypeptide of 396 amino acid residues with a predicted mass of 44,618\u2009Da. The deduced <jats:italic>M. domestica<\/jats:italic> ODC protein was homologous to other ODC proteins. mRNA expression profiles analyzed by real\u2010time PCR indicated that the <jats:italic>ODC<\/jats:italic> transcript is temporally regulated throughout embryogenesis. Sequence data and Southern blot analysis suggests that there were likely only one or two closely linked copies of the <jats:italic>M. domestica ODC<\/jats:italic> gene. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/arch.20442","type":"journal-article","created":{"date-parts":[[2011,9,16]],"date-time":"2011-09-16T14:56:04Z","timestamp":1316184964000},"page":"87-103","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Cloning and molecular characterization of an ornithine decarboxylase gene and its expression during embryonic development of the housefly, <i>Musca domestica<\/i>"],"prefix":"10.1002","volume":"78","author":[{"given":"Michelle J.","family":"Toutges","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adi","family":"Santoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,9,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00300946"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(88)90110-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(90)90371-Q"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00591a005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/35056508"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"24572","DOI":"10.1016\/S0021-9258(19)74505-0","article-title":"Effect of mutations at active site residues on the activity of ornithine decarboxylase and its inhibition by active\u2010site directed irreversible inhibitors","volume":"268","author":"Coleman CS","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-01082-7.50040-0"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.2.1039"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.12.5.2178"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(80)91521-1"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/0968-0004(90)90216-X","article-title":"Molecular genetics of polyamine synthesis in eukaryotic cells","volume":"15","author":"Heby O","year":"1990","journal-title":"Trends Biochem Sci"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.5.1214"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2040771"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(78)90204-X"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00640a033"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(70)90233-7"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91444-H"},{"key":"e_1_2_6_22_1","first-page":"79","volume-title":"Molecular and Cellular Function of the Polyamine","author":"Marton LJ","year":"1987"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1991.tb16410.x"},{"key":"e_1_2_6_24_1","first-page":"759","article-title":"Polyamine metabolism and its importance in neoplastic growth and as a target for chemotherapy","volume":"48","author":"Pegg AE","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1982.243.5.C212"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"8721","DOI":"10.1016\/S0021-9258(18)47475-3","article-title":"Cloning and sequencing of the ornithine decarboxylase gene from Trypanosoma brucei. 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