{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T09:08:43Z","timestamp":1768900123661,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T00:00:00Z","timestamp":1380499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>Lipophorin (<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p) is a major insect lipoprotein and is responsible for lipid transport between organs. In this study, the effect of starvation on <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p properties was analyzed in larval <jats:roman>Manduca sexta<\/jats:roman> during the fifth instar. <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p hemolymph concentrations in larvae at days 1 and 2 were around 2\u20133 mg\/ml and at day 3 it increased to 8 mg\/ml. When larvae were starved for 24 h, they did not grow, but their body mass and hemolymph volume did not decrease significantly. Differences in <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p densities were observed. In fed larvae, from days 1 to 4, two major <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p populations were found with densities of 1.124 \u00b1 0.002 (high density <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p\u2010larval<jats:sub>1<\/jats:sub>, <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>p\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content><jats:sub>1<\/jats:sub>) and 1.141 \u00b1 0.002 g\/ml (<jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>p\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content><jats:sub>2<\/jats:sub>). When larvae were starved for 24 h, only one <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p population was present, with density 1.114 \u00b1 0.001 g\/ml (<jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>p\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content><jats:sub>s<\/jats:sub>). When larvae were abdominally ligated at day 1 or 2 of fifth instar, only <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>p\u2010<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content><jats:sub>s<\/jats:sub> was found after 24 h, indicating that the formation of this <jats:styled-content style=\"fixed-case\">HDL<\/jats:styled-content>p population was not dependent on any factor released by head. On the other hand, larvae that were ligated at day 3 showed the same <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p populations as the fed ones. In 24\u2010h starved larvae, lipid load in <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p was higher as compared to the fed controls. In 24\u2010h ligated larvae <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>p lipid content increased when ligation was performed on day 1 or 2, but not on day 3. So, different responses to starvation can be observed depending on the developmental phase of the same larval instar.<\/jats:p>","DOI":"10.1002\/arch.21133","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:47:15Z","timestamp":1381542435000},"page":"145-156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["EFFECT OF STARVATION ON LIPOPHORIN DENSITY IN FIFTH INSTAR LARVAL <i>Manduca sexta<\/i>"],"prefix":"10.1002","volume":"84","author":[{"given":"Katia C.","family":"Gondim","sequence":"first","affiliation":[{"name":"Instituto de Bioqu\u00edmica M\u00e9dica Universidade Federal do Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E.","family":"Pennington","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Center for Insect Science University of Arizona  Tucson 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