{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:16:32Z","timestamp":1790460992807,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,23]],"date-time":"2015-03-23T00:00:00Z","timestamp":1427068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Priority Academic Program Development of Jiangsu Higher Education Institutions,China"},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31171884"],"award-info":[{"award-number":["31171884"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:p>Insect \u03b3\u2010aminobutyric acid receptors (GABARs) are important molecular targets of cyclodiene and phenylpyrazole insecticides. Previously GABARs encoding <jats:roman>rdl<\/jats:roman> (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. In this study, we cloned the open reading frame cDNA sequence of <jats:roman>rdl<\/jats:roman> gene from fipronil\u2010susceptible and fipronil\u2010resistant strains of <jats:roman>Laodelphax striatellus (Lsrdl)<\/jats:roman>. Sequence analysis confirmed the presence of a previously identified resistance\u2010conferring mutation. Different alternative splicing variants of <jats:roman>Lsrdl<\/jats:roman> were noted. Injection of <jats:roman>dsLsrdl<\/jats:roman> reduced the mRNA abundance of <jats:roman>Lsrdl<\/jats:roman> by 27\u201382%, and greatly decreased fipronil\u2010induced mortality of individuals from both susceptible and resistant strains. These data indicate that <jats:roman>Lsrdl<\/jats:roman> encodes a functional RDL subunit that mediates susceptibility to fipronil. Additionally, temporal and spatial expression analysis showed that <jats:roman>Lsrdl<\/jats:roman> was expressed at higher levels in eggs, fifth\u2010instar nymphs, and female adults than in third\u2010instar and fourth\u2010instar nymphs. <jats:roman>Lsrdl<\/jats:roman> was predominantly expressed in the heads of 2\u2010day\u2010old female adults. All these results provide useful background knowledge for better understanding of fipronil resistance related ionotropic GABA receptor rdl gene expressed variants and potential functional differences in insects.<\/jats:p>","DOI":"10.1002\/arch.21232","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T21:55:52Z","timestamp":1427320552000},"page":"249-261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["KNOCKDOWN OF THE IONOTROPIC \u03b3\u2010AMINOBUTYRIC ACID RECEPTOR (GABAR) <i>RDL<\/i> GENE DECREASES FIPRONIL SUSCEPTIBILITY OF THE SMALL BROWN PLANTHOPPER, <i>Laodelphax striatellus<\/i> (HEMIPTERA: DELPHACIDAE)"],"prefix":"10.1002","volume":"88","author":[{"given":"Qi","family":"Wei","sequence":"first","affiliation":[{"name":"State and Local Joint Engineering Research Center of Green Pesticide Invention and Application Department of Pesticide Sciences College of Plant Protection Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun\u2010Fan","family":"Wu","sequence":"additional","affiliation":[{"name":"State and Local Joint Engineering Research Center of Green Pesticide Invention and Application Department of Pesticide Sciences College of Plant Protection Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Dong","family":"Niu","sequence":"additional","affiliation":[{"name":"State and Local Joint Engineering Research Center of Green Pesticide Invention and Application Department of Pesticide Sciences College of Plant Protection Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua\u2010Yang","family":"Yu","sequence":"additional","affiliation":[{"name":"State and Local Joint Engineering Research Center of Green Pesticide Invention and Application Department of Pesticide 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