{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:19:32Z","timestamp":1761059972069,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,16]],"date-time":"2015-07-16T00:00:00Z","timestamp":1437004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000199","name":"U. S. Department of Agriculture","doi-asserted-by":"crossref","award":["58-0208-0-068"],"award-info":[{"award-number":["58-0208-0-068"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Deployed War-Fighter Protection Research Program"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>A neuronal morphological phenotype can be induced in cultured <jats:italic>Spodoptera frugiperda<\/jats:italic> insect cells (<jats:italic>Sf<\/jats:italic>21) by supplementing serum\u2010containing media with 20\u2010hydroxyecdysone (20\u2010HE) and\/or insulin. In this study, the primary objectives were to determine any role of ion channels in mediating the morphological change in cells treated with 20\u2010HE and insulin, and whether serum was required to observe this effect. Results showed serum\u2010free media also induced growth of processes in Sf21 cells, but at a lower percentage than that found previously in cells bathed in serum\u2010containing media. Veratridine, a sodium channel activator, increased cell survival when applied in combination with 20\u2010HE to <jats:italic>Sf<\/jats:italic>21 cells, and the effect was blocked by tetrodotoxin (1 \u03bcM) a known sodium channel blocker. Cobalt, a calcium channel blocker, showed significant inhibition of cell process growth when applied in combination with both 20\u2010HE and 20\u2010HE plus veratridine. Cobalt also showed significant inhibition of cell process growth when applied in combination with insulin. Thus, some type of sodium channel, as well as a mechanism for transmembrane calcium ion movement, are apparently expressed in <jats:italic>Sf<\/jats:italic>21 cells and are involved in the differentiation process. These cell lines may be used in a wide variety of endeavors, including the screening of insecticides, as well as foster basic studies of neurodevelopment and ecdysone action.<\/jats:p>","DOI":"10.1002\/arch.21249","type":"journal-article","created":{"date-parts":[[2015,7,16]],"date-time":"2015-07-16T12:39:08Z","timestamp":1437050348000},"page":"131-139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["ROLE OF SERUM AND ION CHANNEL BLOCK ON GROWTH AND HORMONALLY\u2010INDUCED DIFFERENTIATION OF <i>Spodoptera frugiperda<\/i> (<i>Sf<\/i>21) INSECT CELLS"],"prefix":"10.1002","volume":"90","author":[{"given":"Lacey J.","family":"Jenson","sequence":"first","affiliation":[{"name":"Neurotoxicology Laboratory Emerging Pathogens Institute Department of Entomology and Nematology University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey R.","family":"Bloomquist","sequence":"additional","affiliation":[{"name":"Neurotoxicology Laboratory Emerging Pathogens Institute Department of Entomology and Nematology University of Florida  Gainesville Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00370946"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(01)00096-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538642"},{"key":"e_1_2_6_5_1","first-page":"85","article-title":"Inhibition by somatostatin of bovine growth hormone secretion following sodium channel activation","volume":"316","author":"Bicknell R","year":"1981","journal-title":"J Phys"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00398084"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00848562"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.877552"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01959903"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10158-006-0036-9"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025708"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.04.030181.000441"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.110.114587"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(80)90491-5"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10158-012-0138-5"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00096.2005"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01260347"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00867801"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(83)90026-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.156.3777.976"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(75)90061-X"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(92)90036-E"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.1440"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5668-03.2004"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402392200"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103713"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm3.1996.377.12.819"},{"key":"e_1_2_6_28_1","unstructured":"WingKD.1995.Anthelmintic N\u2032\u2010substituted\u2010N N\u2032\u2010disubstituted\u2010hydrazines. 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