{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:12:57Z","timestamp":1789384377910,"version":"build-2803163510"},"reference-count":70,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,4,25]],"date-time":"2016-04-25T00:00:00Z","timestamp":1461542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31272036"],"award-info":[{"award-number":["31272036"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31471766"],"award-info":[{"award-number":["31471766"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Doctoral Program of Higher Education of China","award":["20110204110001"],"award-info":[{"award-number":["20110204110001"]}]},{"name":"National Key Technology R&D Program","award":["2012BAK11B03"],"award-info":[{"award-number":["2012BAK11B03"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>The ATP\u2010binding cassette (ABC) transporters are important transmembrane proteins encoded by a supergene family. The majority of ABC proteins are primary active transporters that bind and hydrolyze ATP to mediate the efflux of a diverse range of substrates across lipid membranes. In this study, we cloned and characterized a putative multidrug resistance associated protein 1 (MRP1) from <jats:italic>Rhopalosiphum padi<\/jats:italic> encoded by <jats:italic>ABCC1<\/jats:italic>. Structural analysis showed that this protein has structural features typical of the ABC transporter family. Phylogenetic analysis indicated that the amino acid sequence was highly similar that of the corresponding protein from <jats:italic>Acyrthosiphon pisum<\/jats:italic>. Real\u2010time quantitative polymerase chain reaction (PCR) analysis showed that <jats:italic>ABCC1<\/jats:italic> was expressed throughout all <jats:italic>R. padi<\/jats:italic> developmental stages, with the highest level of expression in the fourth larval instar. We also examined <jats:italic>ABCC1<\/jats:italic> expression in four different tissue types and found that it was most highly expressed in the midgut. Exposing <jats:italic>R. padi<\/jats:italic> to imidacloprid and chlorpyrifos increased <jats:italic>ABCC1<\/jats:italic> expression. Furthermore, <jats:italic>ABCC1<\/jats:italic> expression was higher in the imidacloprid\u2010resistant (IR) and chlorpyrifos\u2010resistant (CR) strains than in an insecticide\u2010susceptible strain (SS) of <jats:italic>R. padi<\/jats:italic>. Exposing <jats:italic>R. padi<\/jats:italic> to verapamil in combination with insecticides significantly increased the toxicity of the insecticides. The respective synergy factor of CR and IR <jats:italic>R. padi<\/jats:italic> strain was 1.33 and 1.26, which was lower than that (2.72 and 1.64, respectively) of the SS. Our results clarify the biological function of <jats:italic>ABCC1<\/jats:italic> in <jats:italic>R. padi<\/jats:italic>, particularly its role in insecticide resistance, and suggest novel strategies for pest management that use ABC transporter inhibitors to increase the effectiveness of insecticides.<\/jats:p>","DOI":"10.1002\/arch.21334","type":"journal-article","created":{"date-parts":[[2016,4,26]],"date-time":"2016-04-26T01:54:40Z","timestamp":1461635680000},"page":"65-84","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["MOLECULAR CLONING, EXPRESSION PATTERN OF MULTIDRUG RESISTANCE ASSOCIATED PROTEIN 1 (MRP1, ABCC1) GENE, AND THE SYNERGISTIC EFFECTS OF VERAPAMIL ON TOXICITY OF TWO INSECTICIDES IN THE BIRD CHERRY\u2010OAT APHID"],"prefix":"10.1002","volume":"92","author":[{"given":"Xin\u2010Le","family":"Kang","sequence":"first","affiliation":[{"name":"Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian\u2010Feng","family":"Qiao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mao\u2010Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Crop Pest Integrated Pest Management on the Loess Plateau of Ministry of Agriculture College of Plant Protection Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2004.03.009"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2011.07.033"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2013.02.008"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/o06-194"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-010-9299-5"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.02.019"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2010.03.004"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001692"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20020957"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2007.12.001"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2002.00365.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/eesa.2002.2186"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153645"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2009.06.005"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1139\/G07-021"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603529200"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-011613-135959"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1360704"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(01)01194-9"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)31588-1"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.12.036"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00035.2005"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2013.11.001"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12052"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1017\/CHOL9780521077910"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.162366399"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2015.01.009"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026796"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2012.05.007"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.08.110192.000435"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(01)00142-1"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113046"},{"key":"e_1_2_5_34_1","first-page":"593","article-title":"Molecular cloning, characterization and expression analysis of ATP\u2010binding cassette transporter genes in the bird cherry\u2010oat aphid, Rhopalosiphum padi (Hemiptera: Aphididae)","volume":"58","author":"Kang XL","year":"2015","journal-title":"Acta Entomologica Sinica"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-14541-4_8"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01911"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2011.11.009"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2004.10.012"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2011.10.001"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-0276140164"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2013.03.009"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-417010-0.00001-X"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02965.x"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2012.00341.x"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-12-46"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00020-09"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2011.03.003"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2012.3089"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-0491(98)10137-2"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2008.00712.x"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10863-007-9115-9"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/eea.12033"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/bse0500179"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1929"},{"key":"e_1_2_5_58_1","first-page":"1449","article-title":"Effect of insecticides and inhibitors on P\u2010glycoprotein ATPase (M\u2010type) activity of resistant pest Helicoverpa armigera","volume":"88","author":"Srinivas R","year":"2005","journal-title":"Curr Sci"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2013.06.010"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr121"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12200"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2011.01490.x"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201411164"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1177\/107327480301000207"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141122891"},{"key":"e_1_2_5_66_1","first-page":"418","volume-title":"The pesticide book","author":"Ware GW","year":"2000"},{"key":"e_1_2_5_67_1","unstructured":"YangF.2013. Molecular cloning and mRNA expression ofHaABCC2gene inHelicoverpa armigera. MSc Thesis. Nanjing: Nanjing Agricultural University."},{"key":"e_1_2_5_68_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi101350x"},{"key":"e_1_2_5_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2011.01097.x"},{"key":"e_1_2_5_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2524"},{"key":"e_1_2_5_71_1","doi-asserted-by":"publisher","DOI":"10.4238\/2015.March.30.26"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Farch.21334","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.21334","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T13:58:40Z","timestamp":1694613520000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.21334"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,25]]},"references-count":70,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1002\/arch.21334"],"URL":"https:\/\/doi.org\/10.1002\/arch.21334","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,25]]},"assertion":[{"value":"2016-03-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}