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In invertebrates, including dipteran and lepidopteran insects, only two isoforms have been reported. 14\u20103\u20103 proteins are scaffold molecules that form homo\u2010 or heterodimeric complexes, acting as molecular adaptors mediating phosphorylation\u2010dependent interactions with signaling molecules involved in immunity, cell differentiation, cell cycle, proliferation, apoptosis, and cancer. Here, we describe the presence of two isoforms of 14\u20103\u20103 in the mosquito <jats:italic>Aedes aegypti<\/jats:italic>, the main vector of dengue, yellow fever, chikungunya, and zika viruses. Both isoforms have the conserved characteristics of the family: two protein signatures (PS1 and PS2), an annexin domain, three serine residues, targets for phosphorylation (positions 58, 184, and 233), necessary for their function, and nine alpha helix\u2010forming segments. By sequence alignment and phylogenetic analysis, we found that the molecules correspond to \u0190 and \u03b6 isoforms (<jats:italic>Aeae<\/jats:italic>14\u20103\u20103\u03b5 <jats:italic>and Aeae<\/jats:italic>14\u20103\u20103\u03b6). The messengers and protein products were present in all stages of the mosquito life cycle and all the tissues analyzed, with a small predominance of <jats:italic>Aeae<\/jats:italic>14\u20103\u20103\u03b6 except in the midgut and ovaries of adult females. The 14\u20103\u20103 proteins in female midgut epithelial cells were located in the cytoplasm. Our results may provide insights to further investigate the functions of these proteins in mosquitoes.<\/jats:p>","DOI":"10.1002\/arch.21348","type":"journal-article","created":{"date-parts":[[2016,9,5]],"date-time":"2016-09-05T11:07:17Z","timestamp":1473073637000},"page":"143-159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["IDENTIFICATION AND EXPRESSION ANALYSIS OF TWO 14\u20103\u20103 PROTEINS IN THE MOSQUITO <i>Aedes aegypti<\/i>, AN IMPORTANT ARBOVIRUSES VECTOR"],"prefix":"10.1002","volume":"93","author":[{"given":"Abel","family":"Trujillo\u2010Ocampo","sequence":"first","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Febe Elena","family":"C\u00e1zares\u2010Raga","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Celestino\u2010Montes","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leticia","family":"Cort\u00e9s\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario H.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Sobre Enfermedades Infecciosas Instituto Nacional de Salud P\u00fablica Cuernavaca  Morelos M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fidel de la Cruz","family":"Hern\u00e1ndez\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional  Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.072280"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2006.03.005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2011.08.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(92)90339-B"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500548"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049671"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0502-e127"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01540-6"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.1.263"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00709-012-0437-z"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-reviews3010"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.40.1.617"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.51.36774"},{"key":"e_1_2_7_15_1","volume-title":"Curr Protoc Cell Biol. 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