{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:46:13Z","timestamp":1787165173163,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T00:00:00Z","timestamp":1535932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008791","name":"Bayer CropScience","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008791","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The full length of\n                    <jats:italic>Culex quiquefasciatus<\/jats:italic>\n                    early trypsin has been cloned and sequenced and a three\u2010dimensional (3D) model of the enzyme was built showing that the enzyme has the canonical trypsin\u2019s active pocket containing H78, D123, S129, and D128. The biosynthesis of juvenile hormone (JH) III by the corpora allata (CA) in female\n                    <jats:italic>Cx. quiquefasciatus<\/jats:italic>\n                    is sugar\u2010dependent. Females that were maintained on water after emergence synthesize very little JH III, JH III bisepoxide, and methyl farnesoate (MF) (3.8, 1.1, and 0.8\u2009fmol\/4\u2009hr\/CA, respectively). One hour after sugar feeding, the synthesis of JH III and JH III bisepoxide reached a maximum (11.3 and 5.9\u2009fmol\/4\u2009hr\/CA, respectively) whereas MF biosynthesis reached a maximum at 24\u2009hr (5.2\u2009fmol\/4\u2009hr\/CA). The early trypsin is transcribed with a short intron (51\u2009nt) is spliced when JH III biosynthesis is high in sugar fed and at 1\u2009hr after the blood meal (22 and 15\u2009 fmol\/4\u2009hr\/CA, respectively). We investigated the transcriptional and posttranscriptional regulation of the early trypsin gene showing that JH III concentrations influence splicing. In the absence JH III the unspliced transcript is linked by a phosphoamide bond at the 5\u2032\u2010end to RNA ribonuleoprotein (RNP). The biosynthesis of the early trypsin was followed in ligated abdomens (without CA) of newly emerged females that fed blood by enema. Our results show that the early trypsin biosynthesis depends on sugar and blood feeding, whereas the late trypsin biosynthesis does not depend on sugar feeding, or JH III biosynthesis. Downregulating the early trypsin transcript does not affect the late trypsin.\n                  <\/jats:p>","DOI":"10.1002\/arch.21506","type":"journal-article","created":{"date-parts":[[2018,9,3]],"date-time":"2018-09-03T10:03:53Z","timestamp":1535969033000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Juvenile hormone affects the splicing of\n                    <i>Culex quinquefasciatus<\/i>\n                    early trypsin messenger RNA"],"prefix":"10.1002","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9880-8910","authenticated-orcid":false,"given":"Dov","family":"Borovsky","sequence":"first","affiliation":[{"name":"Horticultural Research Laboratory USDA\u2010ARS Fort Pierce Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Department of Biology Metropolitan State University of Denver Denver Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Roug\u00e9","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences Pharmaceutiques UMR 152 Pharma\u2010Dev, Universit\u00e9 Toulouse 3 Toulouse Cedex 09 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Powell","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology Indian River Research and Education Center, University of Florida Fort Pierce Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Robert G.","family":"Shatters","sequence":"additional","affiliation":[{"name":"Horticultural Research Laboratory USDA\u2010ARS Fort Pierce Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,3]]},"reference":[{"issue":"14","key":"e_1_2_6_1_2_1","doi-asserted-by":"crossref","first-page":"6739","DOI":"10.1016\/S0021-9258(18)43634-4","article-title":"Purification and properties of a HeLa cell enzyme able to remove the 5\u2032\u2010terminal protein from poliovirus RNA","volume":"255","author":"Ambros V.","year":"1980","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_6_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308133100"},{"key":"e_1_2_6_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(94)00061-L"},{"key":"e_1_2_6_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.142153799"},{"key":"e_1_2_6_1_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq662"},{"key":"e_1_2_6_1_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0985-0"},{"key":"e_1_2_6_1_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/15216540310001597721"},{"key":"e_1_2_6_1_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/29.2.318"},{"key":"e_1_2_6_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(94)90038-8"},{"key":"e_1_2_6_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/29.4.619"},{"key":"e_1_2_6_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0279n.x"},{"key":"e_1_2_6_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.940080406"},{"key":"e_1_2_6_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-470208-0.50015-3"},{"key":"e_1_2_6_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.18.6605"},{"key":"e_1_2_6_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/4.8.2527"},{"key":"e_1_2_6_1_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.2000.00154.x"},{"key":"e_1_2_6_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(91)90050-O"},{"key":"e_1_2_6_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)18026-9"},{"key":"e_1_2_6_1_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr366"},{"key":"e_1_2_6_1_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.11.4683"},{"key":"e_1_2_6_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.1993.tb00127.x"},{"key":"e_1_2_6_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10104"},{"key":"e_1_2_6_1_24_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"},{"key":"e_1_2_6_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(63)90039-8"},{"key":"e_1_2_6_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(69)90251-0"},{"key":"e_1_2_6_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(70)90268-4"},{"key":"e_1_2_6_1_28_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913009050"},{"key":"e_1_2_6_1_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1808146115"},{"key":"e_1_2_6_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.01.011"},{"key":"e_1_2_6_1_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac034989u"},{"key":"e_1_2_6_1_32_1","first-page":"68","article-title":"Modification of substituted seryl and threonyl residues in phosphopeptides and a polysialoglysoprotein by \u03b2\u2010elimination and nucleophile addition","volume":"107","author":"Mega T.","year":"1990","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_6_1_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.1665"},{"key":"e_1_2_6_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05951.x"},{"key":"e_1_2_6_1_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.1996.tb00037.x"},{"key":"e_1_2_6_1_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.1997.00154.x"},{"key":"e_1_2_6_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(99)00052-9"},{"key":"e_1_2_6_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(94)90041-8"},{"key":"e_1_2_6_1_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3162"},{"key":"e_1_2_6_1_40_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20110788"},{"key":"e_1_2_6_1_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc0502210"},{"key":"e_1_2_6_1_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01401-13"},{"key":"e_1_2_6_1_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5362.443"},{"key":"e_1_2_6_1_44_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00411.x"},{"key":"e_1_2_6_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.07.063"},{"key":"e_1_2_6_1_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0216"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Farch.21506","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.21506","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T08:54:41Z","timestamp":1694854481000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.21506"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,3]]},"references-count":45,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/arch.21506"],"URL":"https:\/\/doi.org\/10.1002\/arch.21506","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,3]]},"assertion":[{"value":"2018-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e21506"}}