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In mammals, V\u2010ATPase has been reported to play a key role in cell proliferation and apoptosis. The studies of V\u2010ATPase in silkworm mainly focus on the acidification regulation of midgut and silk gland and immune resistance. However, there are few reports about the function of silkworm V\u2010ATPase on cell proliferation, autophagy, and apoptosis. Thus, the function of V\u2010ATPase in a cell line of <jats:italic>Bombyx mori<\/jats:italic> (BmE) was investigated by treating the cell line with bafilomycin A1, a specific inhibitor of V\u2010ATPase. Cell counting kit 8 (CCK8) and flow cytometry analysis showed that bafilomycin A1 treatment decreased the cell proliferation activity, affected the cell cycle progression and induced cell apoptosis. LysoTracker Red staining showed that the target of bafilomycin A1 is lysosome. The expression of all autophagy\u2010related genes (\n<jats:italic>BmATG5<\/jats:italic>, \n<jats:italic>BmATG6,<\/jats:italic> and \n<jats:italic>BmATG8<\/jats:italic>) decreased, indicating that cell autophagy was inhibited. The analysis of the apoptosis pathway demonstrated that inhibiting the activity of V\u2010ATPase of BmE cells could promote mitochondria to release cytochrome C, inhibit the expression of \n<jats:italic>BmIAP<\/jats:italic>, and activate the caspase cascade to induce apoptosis. All these findings systematically illustrate the effects of V\u2010ATPase on the proliferation, autophagy, and apoptosis in BmE cells, and provide new ideas and a theoretical basis for further study on the function of V\u2010ATPase in BmE.<\/jats:p>","DOI":"10.1002\/arch.21507","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T07:05:23Z","timestamp":1537772723000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Inhibition of silkworm vacuolar\u2010type ATPase activity by its inhibitor Bafilomycin A1 induces caspase\u2010dependent apoptosis in an embryonic cell line of silkworm"],"prefix":"10.1002","volume":"99","author":[{"given":"Xiao\u2010Yin","family":"Tan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Song","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Qian","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing\u2010Qian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Qing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010You","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-0457","authenticated-orcid":false,"given":"Ping","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University Chongqing China"},{"name":"Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,24]]},"reference":[{"issue":"4","key":"e_1_2_8_1_2_1","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1242\/jeb.201.4.479","article-title":"Changes in H+\u2010translocating vacuolar\u2010type ATPase in the anterior silk gland cell of Bombyx mori during metamorphosis","volume":"201","author":"Azuma M.","year":"1998","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.02014"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.7972"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041290110"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2170027"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.138"},{"key":"e_1_2_8_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200111107"},{"key":"e_1_2_8_1_9_1","doi-asserted-by":"publisher","DOI":"10.4137\/JCD.S11034"},{"key":"e_1_2_8_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-011-0675-0"},{"key":"e_1_2_8_1_11_1","volume-title":"Means to an end: apoptosis and other cell death mechanisms","author":"Green D. 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