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One way to improve the efficiency of such methods is through self\u2010limiting, female\u2010eliminating approaches that can alter an insect populations' sex ratio toward males. In this microreview, we summarize recent advances in synthetic sex ratio distorters based on X\u2010chromosome shredding that can induce male\u2010biased progeny. We outline the basic principles to guide the efficient design of an X\u2010shredding system in an XY heterogametic fruit fly species of interest using CRISPR\/Cas gene editing, newly developed computational tools, and insect genetic engineering. We also discuss technical aspects and challenges associated with the efficient transferability of this technology in fruit fly pest populations, toward the potential use of this new class of genetic control approaches for pest management purposes.<\/jats:p>","DOI":"10.1002\/arch.21652","type":"journal-article","created":{"date-parts":[[2019,12,17]],"date-time":"2019-12-17T08:18:55Z","timestamp":1576570735000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Developing CRISPR\u2010based sex\u2010ratio distorters for the genetic control of fruit fly pests: A how to manual"],"prefix":"10.1002","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1890-3067","authenticated-orcid":false,"given":"Konstantina T.","family":"Tsoumani","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biotechnology University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9706-5764","authenticated-orcid":false,"given":"Angela","family":"Meccariello","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Imperial College London  London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2875-5697","authenticated-orcid":false,"given":"Kostas D.","family":"Mathiopoulos","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biotechnology University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8508-4554","authenticated-orcid":false,"given":"Philippos Aris","family":"Papathanos","sequence":"additional","affiliation":[{"name":"Department of Entomology, Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem  Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-10-51"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2018.08.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12592"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2002.2319"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/1-4020-4051-2_10"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-017-9697-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jen.12411"},{"key":"e_1_2_8_9_1","unstructured":"2006 Springer Science & Business Media Dordrecht The Netherlands V. 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