{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T16:04:05Z","timestamp":1776614645710,"version":"3.51.2"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,10]],"date-time":"2020-10-10T00:00:00Z","timestamp":1602288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2017R1A2133009815"],"award-info":[{"award-number":["2017R1A2133009815"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Polytrophic ovarioles of <jats:italic>Spodoptera exigua<\/jats:italic>, a lepidopteran insect, begins with the development of oocytes and differentiation of nurse cells followed by vitellogenesis and choriogenesis. Compared with previtellogenic and vitellogenic developments, choriogenesis has not been clearly understood yet in endocrine control. This study investigated the expression and function of a mucin\u2010like structural protein of <jats:italic>S. exigua<\/jats:italic> called <jats:italic>Se\u2010Mucin1<\/jats:italic> in choriogenesis. It was highly expressed in ovarioles containing chorionated oocytes. The expression level of <jats:italic>Se\u2010Mucin1<\/jats:italic> was increased during adult stage as early as 18\u2009h after adult emergence, reaching the maximal level at 24\u2009h and later. Interestingly, DNA amount of <jats:italic>Se\u2010Mucin1<\/jats:italic> was increased by almost four folds during early adult stage while other genes (<jats:italic>hexokinase<\/jats:italic> and <jats:italic>glyceraldehyde\u20103\u2010phosphate dehydrogenase<\/jats:italic>) not directly associated with chorion formation did not show genomic DNA increase, suggesting specific gene amplification of <jats:italic>Se\u2010Mucin1<\/jats:italic>. RNA interference (RNAi) suppressed <jats:italic>Se\u2010Mucin1<\/jats:italic> expression by injecting 1\u2009\u03bcg of double\u2010strand RNA to teneral females (&lt;5\u2009h after emergence), which exhibited significantly impaired fecundity and egg hatching rate. Eggs laid by RNAi\u2010treated females were malformed in eggshell structures with loss of mesh\u2010like fibers. Treatment with aspirin, a prostaglandin (PG) biosynthesis inhibitor, suppressed the induction of <jats:italic>Se\u2010Mucin1<\/jats:italic> expression during early adult stage and impaired egg development. An addition of PGE<jats:sub>2<\/jats:sub> significantly rescued such impairment in <jats:italic>Se\u2010Mucin1<\/jats:italic> expression and subsequent egg development. These results suggest that PGs mediate choriogenesis of <jats:italic>S. exigua<\/jats:italic> by activating the expression of chorion\u2010associated genes including <jats:italic>Se\u2010Mucin1<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/arch.21748","type":"journal-article","created":{"date-parts":[[2020,10,10]],"date-time":"2020-10-10T14:20:16Z","timestamp":1602339616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["An ovary\u2010specific mucin is associated with choriogenesis mediated by prostaglandin signaling in <i>Spodoptera exigua<\/i>"],"prefix":"10.1002","volume":"106","author":[{"given":"Shabbir","family":"Ahmed","sequence":"first","affiliation":[{"name":"Department of Plant Medicals Andong National University  Andong Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiwon","family":"Seo","sequence":"additional","affiliation":[{"name":"Department of Plant Medicals Andong National University  Andong Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6840-2167","authenticated-orcid":false,"given":"Yonggyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Plant Medicals Andong National University  Andong Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01231"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2019.03.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21625"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16424"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"10660","DOI":"10.1016\/S0021-9258(17)34110-8","article-title":"GATA\u2010type zinc finger motif\u2010containing sequences and chorion gene transcription factors of the silkworm Bombyx mori","volume":"269","author":"Drevet J. 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