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Our previous study has shown that <jats:italic>LmDDX3<\/jats:italic>, the ortholog of <jats:italic>DDX3<\/jats:italic> in <jats:italic>Locusta migratoria<\/jats:italic>, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of <jats:italic>LmDDX3<\/jats:italic> results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterized <jats:italic>LmDDX3<\/jats:italic> expression in female adult locust and analyzed its function in oocyte development. <jats:italic>LmDDX3<\/jats:italic> was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development. <jats:italic>LmDDX3<\/jats:italic> RNAi led to a reduced <jats:italic>vitellogenin<\/jats:italic> (<jats:italic>Vg<\/jats:italic>) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation of <jats:italic>vitellogenin receptor<\/jats:italic> (<jats:italic>VgR<\/jats:italic>) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role for <jats:italic>LmDDX3<\/jats:italic> in regulating the transcription of <jats:italic>Vg<\/jats:italic> and <jats:italic>VgR<\/jats:italic>, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.<\/jats:p>","DOI":"10.1002\/arch.21775","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T06:40:43Z","timestamp":1614580843000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The RNA helicase DDX3 is required for ovarian development and oocyte maturation in <i>Locusta migratoria<\/i>"],"prefix":"10.1002","volume":"106","author":[{"given":"Junxiu","family":"Wang","sequence":"first","affiliation":[{"name":"Research Institute of Applied Biology Shanxi University  Taiyuan Shanxi China"},{"name":"College of Life Science Shanxi University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tingting","family":"Li","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology Shanxi University  Taiyuan Shanxi China"},{"name":"College of Life Science Shanxi University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sufang","family":"Deng","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology Shanxi University  Taiyuan Shanxi China"},{"name":"College of Life Science Shanxi University  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enbo","family":"Ma","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology Shanxi University  Taiyuan Shanxi China"},{"name":"Shanxi Provincial Key Laboratory of Agricultural Integrated Pest Management  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology Shanxi University  Taiyuan Shanxi China"},{"name":"Shanxi Provincial Key Laboratory of Agricultural Integrated Pest Management  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9984-3419","authenticated-orcid":false,"given":"Shuping","family":"Xing","sequence":"additional","affiliation":[{"name":"Research Institute of Applied Biology Shanxi University  Taiyuan Shanxi China"},{"name":"Shanxi Provincial Key Laboratory of Agricultural Integrated Pest Management  Taiyuan Shanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00423"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.183723"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms160818368"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90432-5"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh240"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects10120445"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7322(93)90016-T"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1080010408"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/des047"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201801068R"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01532"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12646"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003085"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.07.109"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.005692"},{"issue":"1","key":"e_1_2_9_17_1","first-page":"92","article-title":"Belle is a Drosophila DEAD\u2010box protein required for viability and in the germ line. 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