{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T15:42:54Z","timestamp":1784907774284,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,7,1]],"date-time":"2021-07-01T00:00:00Z","timestamp":1625097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to develop a new control method for <jats:italic>Drosophila<\/jats:italic> using saccharin sodium dihydrate (saccharin), an artificial sweetener that is safe for humans and the environment, and to elucidate its mode of action. In this study, we confirmed that saccharin can dose\u2010dependently inhibit the development of or kill vinegar flies (VFs) and spotted wing <jats:italic>Drosophila<\/jats:italic> (SWDs). In addition, we found that low concentrations of saccharin induced a similar effect as starvation in <jats:italic>Drosophila<\/jats:italic>, whereas high concentrations of saccharin induced changes in the unfolded protein response (UPR) and autophagy signaling that were unlike starvation and inhibited development or killed the VF and the SWD by performing real\u2010time quantitative polymerase chain reaction analyses. Spinosad is a widely used plant protection agent for SWD control. When saccharin was cotreated with 0.25\u20131.0\u2009ppm spinosad, an additive insecticidal activity was observed only at high concentrations of saccharin. However, when saccharin was cotreated with 2.0 ppm spinosad, an additive insecticidal activity was observed at low concentrations of saccharin. Taken together, alteration of UPR and autophagy signaling represented the molecular basis underlying saccharin toxicity to <jats:italic>Drosophila<\/jats:italic> and concurrent spraying of an insecticide with saccharin could enhance the insecticidal activities.<\/jats:p>","DOI":"10.1002\/arch.21826","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T09:38:59Z","timestamp":1625218739000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Alteration of unfolded protein responses and autophagy signaling represented the molecular basis underlying saccharin toxicity to <i>Drosophila<\/i> (Diptera: Drosophilidae)"],"prefix":"10.1002","volume":"107","author":[{"given":"Angelina F.","family":"Osabutey","sequence":"first","affiliation":[{"name":"Department of Bio\u2010medical Gerontology Hallym University Graduate School Chuncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A\u2010Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Ilsong Institute of Life Sciences Hallym University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo Yoon","family":"Seo","sequence":"additional","affiliation":[{"name":"Crop Protection Division National Institute of Agricultural Sciences Wanju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linh Xuan","family":"Mai","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010medical Gerontology Hallym University Graduate School Chuncheon Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3608-4376","authenticated-orcid":false,"given":"Young Ho","family":"Koh","sequence":"additional","affiliation":[{"name":"Department of Bio\u2010medical Gerontology Hallym University Graduate School Chuncheon Republic of Korea"},{"name":"Ilsong Institute of Life Sciences Hallym University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085356"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0098949"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/su12176963"},{"key":"e_1_2_9_5_1","first-page":"2646","volume-title":"Encyclopedia of entomology","author":"Behmer S. T.","year":"2008"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2017.04.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5240"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2017.303"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fagro.2019.00002"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0171848"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21514"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1518-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aspen.2016.10.015"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.5656\/KSAE.2015.05.0.020"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.5656\/KSAE.2013.08.0.038"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuw032"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1208887"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/tow019"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/01677063.2012.694931"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.117.300583"},{"key":"e_1_2_9_21_1","first-page":"14","article-title":"The metabolism and toxicology of saccharin","volume":"1","author":"Okoduwa S. I. R.","year":"2013","journal-title":"InfoHealth Awareness Article"},{"key":"e_1_2_9_22_1","unstructured":"RDA. (2020).Guide to the correct use of pesticides by item.http:\/\/www.nongsaro.go.kr\/portal\/ps\/psz\/psza\/contentMain.ps?menuId=PS65146"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toy274"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects10070196"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2015.10.012"},{"key":"e_1_2_9_27_1","unstructured":"Zalom F. G. Bolda M. P. Dara S. K. &Joseph S. V.(2018).Agriculture: Strawberry pest management guidelines vinegar flies: Drosophila melanogaster and other species.https:\/\/www2.ipm.ucanr.edu\/agriculture\/strawberry\/Vinegar-Fly\/"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083714"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.21826","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/arch.21826","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.21826","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T22:02:54Z","timestamp":1693346574000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.21826"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1002\/arch.21826"],"URL":"https:\/\/doi.org\/10.1002\/arch.21826","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7]]},"assertion":[{"value":"2021-04-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e21826"}}