{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T23:46:04Z","timestamp":1769039164944,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772680"],"award-info":[{"award-number":["31772680"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072795"],"award-info":[{"award-number":["32072795"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Phosphoacetyl glucosamine mutase (PGM) is the key gene for glycolysis of important metabolic pathways in silkworm, and H<jats:sub>2<\/jats:sub>S (7.5\u2009\u03bcM) can promote the growth and development of silkworm. Herein, we used body cavity injection of small\u2010interfering RNA\u00a0(siRNA) to interfere with the PGM gene in H<jats:sub>2<\/jats:sub>S\u2010treated silkworms. After RNA interference (RNAi), we investigated the growth and development of the silkworm. H<jats:sub>2<\/jats:sub>S treatment could significantly recover the inhibition of body weight, cocoon weight, cocoon shell weight, and cocoon shell ratio by knocking down PGM gene in silkworm, without significant effects on eggs laying and production, and then analyzed the mRNA expression of PGM gene. The interference of siRNA significantly decreased the expression of targeted PGM gene and was concentrated in 48\u2009h followed by gradual recovery. Three interference fragments also showed different interference effects, and siRNA of PGM\u20103 exerted the highest interference effect to the target gene expression. Fat body had the highest mRNA expression of PGM gene, and the best interference effect was observed after siRNA injection. The results showed that the gene based on H<jats:sub>2<\/jats:sub>S treatment may have an important impact on the growth and development of silkworm by affecting its metabolic pathway.<\/jats:p>","DOI":"10.1002\/arch.21873","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T07:58:44Z","timestamp":1643875124000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hydrogen sulfide treatment retrieves the inhibition of growth and development characteristics in silkworm (<i>Bombyx mori<\/i>) <i>via<\/i> phosphoacetyl glucosamine mutase gene knock down"],"prefix":"10.1002","volume":"110","author":[{"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology Hefei PR 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Biotechnology Jiangsu University of Science and Technology Zhenjiang PR China"},{"name":"Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture, Sericultural Research Institute Chinese Academy of Agricultural Sciences Zhenjiang PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology Hefei PR China"},{"name":"School of Biological Science and Engineering North Minzu University Yinchuan PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1729-209X","authenticated-orcid":false,"given":"Zhao\u2010Jun","family":"Wei","sequence":"additional","affiliation":[{"name":"School of Food and Biological Engineering Hefei University of Technology Hefei PR China"},{"name":"School of Biological Science and Engineering North Minzu University Yinchuan PR 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