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Northern blot analyses show that the three transcripts are expressed in the head thorax, the gut, the ovaries, and the fat body of females. Molecular modeling shows that the enzyme is a homodimer that binds JH\u00a0III acid (JH IIIA) at the catalytic groove better than JH III. The cDNA of JHEH 1 and 2 are very similar indicating close relationship. Knocking down of <jats:italic>jheh<\/jats:italic> 1, 2, and 3 in adult female and larval <jats:italic>Ae. aegypti<\/jats:italic> using double\u2010stranded RNA\u00a0(dsRNA) did not affect egg development or caused adult mortality. Larvae that were fed bacterial cells expressing dsRNA against <jats:italic>jheh<\/jats:italic> 1, 2, and 3 grew normally. Treating blood\u2010fed female <jats:italic>Ae. aegypti<\/jats:italic> with [12\u2010<jats:sup>3<\/jats:sup>H](10R) JH III and analyzing the metabolites by C<jats:sub>18<\/jats:sub> reversed phase chromatography showed that JHEH preferred substrate is not JH III but JH IIIA. Genomic analysis of <jats:italic>jheh<\/jats:italic> 1, 2, and 3 indicate that <jats:italic>jheh<\/jats:italic> 1 and 2 are transcribed from a 1.53\u2009kb DNA whereas <jats:italic>jheh<\/jats:italic> 3 is transcribed from a 10.9\u2009kb DNA. All three genes are found on chromosome two at distinct locations. JHEH 2 was expressed in bacterial cells and purified by Ni affinity chromatography. Sequencing of the recombinant protein by MS\/MS identified JHEH 2 as the expressed recombinant protein.<\/jats:p>","DOI":"10.1002\/arch.21977","type":"journal-article","created":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T10:25:50Z","timestamp":1666088750000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cloning and characterization of <i>Aedes aegypti<\/i> juvenile hormone epoxide hydrolases (JHEHs)"],"prefix":"10.1002","volume":"112","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9880-8910","authenticated-orcid":false,"given":"Dov","family":"Borovsky","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Genetics University of Colorado Anschutz Medical Campus Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelien","family":"Van Ekert","sequence":"additional","affiliation":[{"name":"Syngenta Crop Protection LLC  North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Buytaert","sequence":"additional","affiliation":[{"name":"Zoological Institute KU Leuven Leuven Flanders Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Peeters","sequence":"additional","affiliation":[{"name":"Open BioLab Brussels Erasmushogeschool Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Roug\u00e9","sequence":"additional","affiliation":[{"name":"Faculte des Sciences Pharmaceutiques Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801559105"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2004.12.008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti770"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq662"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/29.4.619"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1996.0279n.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(92)90045-G"},{"key":"e_1_2_8_10_1","first-page":"16","article-title":"All\u2010atom structure validation for macromolecular crystallography","volume":"66","author":"Chenn V. 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