{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:22:31Z","timestamp":1789755751757,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["2018\u201005974"],"award-info":[{"award-number":["2018\u201005974"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Colorado potato beetle (<jats:italic>Leptinotarsa decemlineata<\/jats:italic> (Say)) is an insect pest that threatens potato crops. Multiple options exist to limit the impact of this pest even though insecticides remain a primary option for its control. Insecticide resistance has been reported in Colorado potato beetles and a better understanding of the molecular players underlying such process is of utmost importance to optimize the tools used to mitigate the impact of this insect. Resistance against the insecticide spinosad has been reported in this insect and this work thus aims at exploring the expression of targets previously associated with insecticide response in Colorado potato beetles exposed to this compound. Amplification and quantification of transcripts coding for cytochrome P450s and glutathione S\u2010transferases were conducted via qRT\u2010PCR in insects treated with varying doses of spinosad and for different time duration. This approach notably revealed differential expression of <jats:italic>CYP6a23<\/jats:italic> and <jats:italic>CYP12a5<\/jats:italic> in insects exposed to low doses of spinosad for 4\u2009h\u00a0as well as modulation of <jats:italic>CYP6a13<\/jats:italic>, <jats:italic>CYP6d4<\/jats:italic>, <jats:italic>GST<\/jats:italic>, <jats:italic>GST1<\/jats:italic>, and <jats:italic>GST1\u2010Like<\/jats:italic> in insects treated with high doses of spinosad for the same duration. RNAi\u2010based targeting of <jats:italic>CYP4g15<\/jats:italic> and <jats:italic>CYP6a23<\/jats:italic> was associated with marked reduction of transcript expression 7\u00a0days following dsRNA injection and reduction of the former had a marked impact on insect viability. In general, results presented here provide novel information regarding the expression of transcripts relevant to spinosad response in Colorado potato beetles and reveal a novel target to consider in the development of RNAi\u2010based strategies aimed at this potato pest.<\/jats:p>","DOI":"10.1002\/arch.21993","type":"journal-article","created":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T10:35:24Z","timestamp":1671705324000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Spinosad\u2010associated modulation of select cytochrome P450s and glutathione S\u2010transferases in the Colorado potato beetle, <i>Leptinotarsa decemlineata<\/i>"],"prefix":"10.1002","volume":"112","author":[{"given":"Pierre","family":"Bastarache","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Universit\u00e9 de Moncton  Moncton New Brunswick 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