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With a current over\u2010dependence on chemical insecticides in the control of insect pests, leading to increased chemical resistance in target organisms, as well as side effects on nontarget organisms, the wider environment, and human health, finding alternative solutions is paramount. The employment of entomopathogenic fungi is one such potential avenue in the pursuit of greener, more target\u2010specific methods of insect pest control. To this end, the present study tested the chemical constituents of <jats:italic>Metarhizium anisopliae<\/jats:italic> fungi against the unicellular protozoan malaria parasite <jats:italic>Plasmodium falciparum<\/jats:italic>, the insect pests <jats:italic>Anopheles stephensi<\/jats:italic> Listen, <jats:italic>Spodoptera litura<\/jats:italic> Fabricius, and <jats:italic>Tenebrio molitor<\/jats:italic> Linnaeus, as well as the nontarget bioindicator species, <jats:italic>Eudrilus eugeniae<\/jats:italic> Kinberg. Fungal crude chemical molecules caused a noticeable anti\u2010plasmodial effect against <jats:italic>P. falciparum<\/jats:italic>, with IC<jats:sub>50<\/jats:sub> and IC<jats:sub>90<\/jats:sub> values of 11.53 and 7.65\u2009\u00b5g\/mL, respectively. The crude chemical molecules caused significant larvicidal activity against insect pests, with LC<jats:sub>50<\/jats:sub> and LC<jats:sub>90<\/jats:sub> values of 49.228\u221271.846\u2009\u00b5g\/mL in <jats:italic>A. stephensi<\/jats:italic>, 32.542\u221276.510\u2009\u00b5g\/mL in <jats:italic>S. litura<\/jats:italic>, and 38.503\u221288.826\u2009\u00b5g\/mL in <jats:italic>T. molitor<\/jats:italic> at 24\u2009h posttreatment. Based on the results of the nontarget bioassay, it was revealed that the fungal\u2010derived crude extract exhibited no histopathological sublethal effects on the earthworm <jats:italic>E. eugeniae<\/jats:italic>. LC\u2010MS analysis of <jats:italic>M. anisopliae\u2010<\/jats:italic>derived crude metabolites revealed the presence of 10 chemical constituents. Of these chemicals, three major chemical constituents, namely, camphor (15.91%), caprolactam (13.27%), and monobutyl phthalate (19.65%), were highlighted for potential insecticidal and anti\u2010malarial activity. The entomopathogenic fungal\u2010derived crude extracts thus represent promising tools in the control of insect pests and malarial parasites.<\/jats:p>","DOI":"10.1002\/arch.22037","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T11:09:32Z","timestamp":1690456172000},"page":"1-19","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Biocontrol effect of entomopathogenic fungi <i>Metarhizium anisopliae<\/i> ethyl acetate\u2010derived chemical molecules: An eco\u2010friendly anti\u2010malarial drug and insecticide"],"prefix":"10.1002","volume":"114","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3283-1306","authenticated-orcid":false,"given":"Vivekanandhan","family":"Perumal","sequence":"first","affiliation":[{"name":"Department of General Pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences Saveetha University Chennai Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swathy","family":"Kannan","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology, Faculty of Agriculture Chiang Mai University Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Alford","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, Life Sciences Building University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarayut","family":"Pittarate","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology, Faculty of Agriculture Chiang Mai University Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Supamit","family":"Mekchay","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences, Faculty of Agriculture Chiang Mai University Chiang Mai Thailand"},{"name":"Innovative Agriculture Research Center, Faculty of Agriculture Chiang Mai University Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gadi V. 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