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To control this pest, a novel strategy to suppress the gene(s) associated with sexually dimorphic phenotypes has been devised and implemented in a laboratory scale. However, comprehensive transcriptomic analysis related to this sex differentiation of <jats:italic>Z. scutellata<\/jats:italic> was necessary to determine effective target genes for the genetic control. We performed de novo assembly of the transcript obtained by paired\u2010end sequencing using an Illumina HiSeq platform and let to 217,967 unigenes (i.e., unique genes) with a minimum length of 200\u2009bp. The female produced 31, 604, 442 reads with 97.93% of Q20, 94.76% of Q30, and the male produced 130, 592, 828 reads with 97.93% of Q20 and 94.76 of Q30%. The differentially expressed genes were used to predict genetic factors associated with sex differentiation, which included <jats:italic>Rho1, extra\u2010macrochaetae<\/jats:italic> (<jats:italic>emc<\/jats:italic>), <jats:italic>hopscotch<\/jats:italic> (<jats:italic>hop<\/jats:italic>), <jats:italic>doublesex<\/jats:italic> (<jats:italic>dsx<\/jats:italic>), <jats:italic>sex\u2010lethal<\/jats:italic> (<jats:italic>sxl<\/jats:italic>), <jats:italic>transformer\u20102<\/jats:italic> (<jats:italic>tra\u20102<\/jats:italic>), <jats:italic>testis\u2010specific serine\/threonine\u2010protein kinase<\/jats:italic> (<jats:italic>tssk1<\/jats:italic>), <jats:italic>tektin1<\/jats:italic> (<jats:italic>tkt1<\/jats:italic>) and <jats:italic>2<\/jats:italic> (<jats:italic>tkt2<\/jats:italic>), <jats:italic>odorant binding proteins<\/jats:italic> (<jats:italic>OBP<\/jats:italic>s), <jats:italic>fruitless<\/jats:italic> (<jats:italic>fru<\/jats:italic>), <jats:italic>vitellogenin receptor<\/jats:italic>, and <jats:italic>hormone receptors<\/jats:italic> in <jats:italic>Z. scutellata<\/jats:italic>. In addition, this transcriptome analysis provides the additional gene associated with sex determination and mating behaviors, which would be applied to develop a novel sterile insect technique against <jats:italic>Z. scutellata<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/arch.22064","type":"journal-article","created":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T12:03:53Z","timestamp":1699272233000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Insights on reproduction\u2010related genes in the striped fruit fly, <i>Zeugodacus scutellata<\/i> (Hendel) (Diptera: Tephritidae)"],"prefix":"10.1002","volume":"115","author":[{"given":"Anandapadmanaban","family":"Gokulanathan","sequence":"first","affiliation":[{"name":"Plant Quarantine Technology Center Animal and Plant Quarantine Agency Gimcheon South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyoung\u2010ho","family":"Mo","sequence":"additional","affiliation":[{"name":"Plant Quarantine Technology Center Animal and Plant Quarantine Agency Gimcheon South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youngjin","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Plant Medicals Andong National University Andong South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects11040211"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh131"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000356458"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01101-x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2022.884159"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12041-010-0039-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2005.07.006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects9030096"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00239-004-0241-2"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41437-022-00536-y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.9438"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/aen.12365"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-015559-3.50005-X"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jia.2022.08.007"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1223"},{"issue":"5","key":"e_1_2_9_18_1","first-page":"294","article-title":"DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates","volume":"3","author":"Folmer O.","year":"1994","journal-title":"Molecular Marine Biology and Biotechnology"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201000043"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805993105"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-6055.1993.tb00561.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19113691"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-815160-0.00003-7"},{"key":"e_1_2_9_24_1","unstructured":"Jeon S.W.(2011)Biological characteristics and seasonal occurrence of Bactrocera scutellata[PhD Thesis]. 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