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Despite the economic impact and widespread distribution of<jats:italic>C. hemipterus<\/jats:italic>, there is a lack of functional genomics research seeking to elucidate features of molecular physiology for improved pest management. Here, we report the characterization of the gene named<jats:italic>Vermilion<\/jats:italic>in<jats:italic>C. hemipterus<\/jats:italic>(<jats:italic>ChVer<\/jats:italic>) that encodes for tryptophan 2,3\u2010dioxygenase. The<jats:italic>Vermilion<\/jats:italic>is frequently used as a visual marker for genomics approaches as tryptophan 2,3\u2010dioxygenase is involved in the biosynthesis of eye coloration pigments in insects. We identified 1628\u2009bp long full\u2010length transcript of<jats:italic>ChVer<\/jats:italic>from transcriptomic database of<jats:italic>C. hemipterus<\/jats:italic>. The expression analysis among adult body parts revealed peak<jats:italic>ChVer<\/jats:italic>expression in head compared to thorax and abdomen, which is consistent with its role. Among the<jats:italic>C. hemipterus<\/jats:italic>developmental stages, peak<jats:italic>ChVer<\/jats:italic>expression was observed in first instar larva, second instar larva, and adult male stages, whereas the lowest levels of expression were seen in third instar larva, prepupa, and pupa. The nanoinjection of<jats:italic>ChVer<\/jats:italic>double\u2010stranded RNA\u00a0in larval<jats:italic>C. hemipterus<\/jats:italic>resulted in a significant reduction in<jats:italic>ChVer<\/jats:italic>transcript levels as well as caused a loss of eye color, that is, the white\u2010eyed phenotype in adults. Characterization of visually traceable marker gene and robust RNA interference\u00a0response seen in this study will enable genomics research is this important pest.<\/jats:p>","DOI":"10.1002\/arch.22072","type":"journal-article","created":{"date-parts":[[2023,12,19]],"date-time":"2023-12-19T14:09:10Z","timestamp":1702994950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization and RNA interference\u2010mediated silencing of tryptophan 2,3\u2010dioxygenase gene in<i>Carpophilus hemipterus<\/i>(L.) (Coleoptera: Nitidulidae)"],"prefix":"10.1002","volume":"115","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7133-0785","authenticated-orcid":false,"given":"Raman","family":"Bansal","sequence":"first","affiliation":[{"name":"USDA\u2010ARS San Joaquin Valley Agricultural Sciences Center Parlier California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaoyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS National Soil Dynamics Research Laboratory Auburn Alabama USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles S.","family":"Burks","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS San Joaquin Valley Agricultural Sciences Center Parlier California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Spencer S.","family":"Walse","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS San Joaquin Valley Agricultural Sciences Center Parlier California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne B.","family":"Hunter","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS U.S. Horticultural Research Laboratory Fort Pierce Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-020-01167-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","unstructured":"Bansal R. Burks C. Walse S.(2023) Gene expression patterns to identify stable reference genes for developmental and gender expression analyses inCarpophilus hemipterus(L.) 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