{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T18:24:31Z","timestamp":1766946271726,"version":"3.41.2"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spotted\u2010wing drosophila, <jats:italic>Drosophila suzukii<\/jats:italic> (Matsumura), is an invasive vinegar fly that is a major threat to the small fruits industries globally. Insect <jats:italic>capa<\/jats:italic> genes encode multiple neuropeptides, including CAPA\u2010periviscerokinin (CAPA\u2010PVK) peptides, that are specifically known to cause diuresis or anti\u2010diuresis in various organisms. Here we identified and characterized a corresponding G protein\u2010coupled receptor (GPCR) of the <jats:italic>D. suzukii<\/jats:italic> CAPA\u2010PVK peptides: CAPA receptor (CAPA\u2010R). To better characterize the behavior of <jats:italic>D. suzukii<\/jats:italic> CAPA\u2010R, we used insect cell\u2010based functional expression assays to evaluate responses of CAPA\u2010R\u00a0against\u00a0<jats:italic>D. suzukii<\/jats:italic> CAPA\u2010PVKs, CAPA\u2010PVKs from five species in Insecta, one species from Mollusca, modified CAPA\u2010PVK peptides, and some PRXamide family peptides: pyrokinin (PK), diapause hormone (DH), and ecdysis\u2010triggering hormone (ETH). Functional studies revealed that the <jats:italic>D. suzukii<\/jats:italic> CAPA\u2010R is strongly activated by both of its own natural <jats:italic>D. suzukii<\/jats:italic> CAPA\u2010PVKs, and interestingly, it was strongly activated by other CAPA\u2010PVK peptides from <jats:italic>Frankliniella occidentallis<\/jats:italic> (Thysanoptera), <jats:italic>Solenopsis invicta<\/jats:italic> (Hymenoptera), <jats:italic>Helicoverpa zea<\/jats:italic> (Lepidoptera) and <jats:italic>Plutella xylostella<\/jats:italic> (Lepidoptera). However, <jats:italic>D. suzukii<\/jats:italic> CAPA\u2010R was not activated by Mollusca CAPA\u2010PVK or the other PRXamide peptides. Gene expression analyses showed that the CAPA\u2010R was highly expressed in the Malpighian tubules and moderately in hindgut compared to other digestive organs or the rest of body, supporting diuretic\/antidiuretic functionality. When compared across life stages of <jats:italic>D. suzukii<\/jats:italic>, expression of CAPA\u2010R was approximately 1.5x greater in the third instar than the other stages and minimally detected in the eggs, 4\u2010day old pupae and 3\u2010day old adults. Our results functionally characterized the <jats:italic>D. suzukii<\/jats:italic> CAPA\u2010R and a few short peptides were identified as potential biological targets to exploit the CAPA\u2010R for <jats:italic>D. suzukii<\/jats:italic> management.<\/jats:p>","DOI":"10.1002\/arch.22080","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T07:24:56Z","timestamp":1723793096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Functional expression and characterization of CAPA receptor in the digestive tract and life stages of <i>Drosophila suzukii<\/i>, and differential activities with insect PRXamide peptides"],"prefix":"10.1002","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1962-8431","authenticated-orcid":false,"given":"Briana E.","family":"Price","sequence":"first","affiliation":[{"name":"USDA\u2010ARS, Horticultural Crops Disease and Pest Management Research Unit Corvallis Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0460-9924","authenticated-orcid":false,"given":"Hyo Sang","family":"Jang","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS, Horticultural Crops Disease and Pest Management Research Unit Corvallis Oregon USA"},{"name":"Department of Horticulture Oregon State University Corvallis Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8141-7300","authenticated-orcid":false,"given":"Ryssa K.","family":"Parks","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS, Horticultural Crops Disease and Pest Management Research Unit Corvallis Oregon USA"},{"name":"Department of Horticulture Oregon State University Corvallis Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0769-380X","authenticated-orcid":false,"given":"Man\u2010Yeon","family":"Choi","sequence":"additional","affiliation":[{"name":"USDA\u2010ARS, Horticultural Crops Disease and Pest Management Research Unit Corvallis Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00559"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-015-0681-z"},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","unstructured":"Atallah J. Teixeira L. Salazar R. Zaragoza G.&Kopp A.(2014)\u00a0The making of a pest: the evolution of a fruit\u2010penetrating ovipositor inDrosophila suzukiiand related species.Proceedings of the Royal Society Biological Sciences 281 20132840.https:\/\/doi.org\/10.1098\/rspb.2013.2840","DOI":"10.1098\/rspb.2013.2840"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-204X2016000500018"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2017.01.011"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1632485100"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.12.004"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.02.033"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11040583"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-001-0467-x"},{"volume-title":"Current protocols in protein science","year":"1996","author":"Coligan J.E.","key":"e_1_2_9_12_1"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2013.03.012"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2021.170610"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jipm\/pmz022"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410051222"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4497"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5971"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7800"},{"key":"e_1_2_9_20_1","doi-asserted-by":"crossref","unstructured":"Hallgren J. Tsirigos K.D. Pedersen M.D. Armenteros J.J.A. Marcatili P.&Nielsen H.\u00a0et al. 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Park Y. Nachman R.J.&Orchard I.(2010)\u00a0Isolation expression analysis and functional characterization of the first antidiuretic hormone receptor in insects.Proceedings of the National Academy of Sciences in the USA. 107(22) \u00a010290\u221210295.https:\/\/doi.org\/10.1073\/pnas.1003666107","DOI":"10.1073\/pnas.1003666107"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2011.12.022"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.162276199"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(94)00144-U"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-006-6187-3"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3233\/JBR-150092"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-58731-y"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-031616-035500"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12951"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-7-1551"},{"key":"e_1_2_9_45_1","doi-asserted-by":"crossref","unstructured":"Terhzaz S. Teets N.M. Cabrero P. Henderson L. Ritchie M.G.&Nachman R.J.\u00a0et al. (2015) Insect capa neuropeptides impact desiccation and cold tolerance.Proceedings of the National Academy of Sciences in the USA. 112(9) \u00a02882\u22122887.https:\/\/doi.org\/10.1073\/pnas.1501518112","DOI":"10.1073\/pnas.1501518112"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.114.1.365"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.114.1.381"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.116.1.395"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02615077"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/jen.12200"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.826399"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2021.170665"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12859"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.22080","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,3]],"date-time":"2024-09-03T04:44:39Z","timestamp":1725338679000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.22080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8]]},"references-count":52,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1002\/arch.22080"],"URL":"https:\/\/doi.org\/10.1002\/arch.22080","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"type":"print","value":"0739-4462"},{"type":"electronic","value":"1520-6327"}],"subject":[],"published":{"date-parts":[[2024,8]]},"assertion":[{"value":"2023-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e22080"}}