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Besides its important role in the reproductive pathway, Vg occurs in nonreproductive worker honey bee, suggesting its participation in other pathways. The objective was to verify if the VgR occurs in the hypopharyngeal glands of <jats:italic>Apis mellifera<\/jats:italic> workers and how Vg is internalized by these cells. VgR occurrence in the hypopharyngeal glands was evaluated by qPCR analyses of VgR and immunohistochemistry in workers with different tasks. The VgR gene is expressed in the hypopharyngeal glands of workers with higher transcript levels in nurse honey bees. VgR is more expressed in 11\u2010day\u2010old workers from queenright colonies, compared to orphan ones. Nurse workers with developed hypopharyngeal glands present higher VgR transcripts than those with poorly developed glands. The immunohistochemistry results showed the co\u2010localization of Vg, VgR and clathrin (protein that plays a major role in the formation of coated vesicles in endocytosis) in the hypopharyngeal glands, suggesting receptor\u2010mediated endocytosis. The results demonstrate that VgR performs the transport of Vg to the hypopharyngeal glands, supporting the Ovary Ground Plan Hypothesis and contributing to the understanding of the role of this gland in the social context of honey bees.<\/jats:p>","DOI":"10.1002\/arch.22120","type":"journal-article","created":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T18:28:20Z","timestamp":1715624900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Expression and function of the vitellogenin receptor in the hypopharyngeal glands of the honey bee <i>Apis mellifera<\/i> (Hymenoptera: Apidae) workers"],"prefix":"10.1002","volume":"116","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9562-7310","authenticated-orcid":false,"given":"Virginia Teles","family":"Dohanik","sequence":"first","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5380-9864","authenticated-orcid":false,"given":"Luanda","family":"Medeiros\u2010Santana","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade Universidade Federal de Vi\u00e7osa, Campus Rio Parana\u00edba  Rio Parana\u00edba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8538-4303","authenticated-orcid":false,"given":"Carolina Gon\u00e7alves","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0304-7002","authenticated-orcid":false,"given":"Weyder Cristiano","family":"Santana","sequence":"additional","affiliation":[{"name":"Departamento de Entomologia Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0477-4252","authenticated-orcid":false,"given":"Jos\u00e9 Eduardo","family":"Serr\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Geral Universidade Federal de Vi\u00e7osa  Vi\u00e7osa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,13]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"The molecular biology of the cell","author":"Alberts B.","year":"2015"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/518183"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0333979100"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2006.01.006"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/jtbi.2002.2545"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(03)00121-8"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-3-1"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2004.02.010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-52789-z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2011.04.015"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","unstructured":"Barbosa P. 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