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Previous research has demonstrated that short\u2010term high temperatures induce differential expression of the vitellogenin receptor (<jats:italic>ZcVgR<\/jats:italic>) gene, reducing the number of eggs laid and the lifespan of female <jats:italic>Z. cucurbitae<\/jats:italic>. In this paper, we used Tandem Mass Tags (TMT) quantitative proteomics and Illumina high\u2010throughput sequencing to determine the proteomic and transcriptomic information of female <jats:italic>Z. cucurbitae<\/jats:italic> after siRNA\u2010mediated silencing of the target gene (<jats:italic>ZcVgR<\/jats:italic>) to gain a comprehensive understanding of the molecular mechanism of this gene in the regulation of reproduction and lifespan. The findings demonstrated that following the target gene's silencing, the <jats:italic>ZcVgR<\/jats:italic> gene's transcriptional expression was significantly downregulated, and there was no significant difference in protein level. The transcriptome and proteome had a low correlation; when the <jats:italic>ZcVgR<\/jats:italic> gene was silenced, <jats:italic>vitellogenin\u20101<\/jats:italic> (<jats:italic>ZcVg1<\/jats:italic>), <jats:italic>juvenile hormone epoxide hydrolase<\/jats:italic> (<jats:italic>JHEH<\/jats:italic>), <jats:italic>troponin C<\/jats:italic> (<jats:italic>TnC<\/jats:italic>), <jats:italic>heat shock protein 70<\/jats:italic> (<jats:italic>HSP70<\/jats:italic>), and other related genes were downregulated at the transcriptional level. By silencing the <jats:italic>ZcVgR<\/jats:italic> gene, transcriptionally level immune\u2010related pathways were activated and energy metabolism\u2010related pathways were inhibited; protein\u2010level glycometabolism and phagosome pathways were activated, while phototransduction\u2010fly and autophagy\u2010animal pathways were inhibited. The findings of this study might offer a theoretical foundation for integrated management of <jats:italic>Z. cucurbitae<\/jats:italic> in the summertime.<\/jats:p>","DOI":"10.1002\/arch.70015","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T18:25:00Z","timestamp":1734459900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Knockdown of the <i>ZcVgR<\/i> Gene Alters the Expression of Genes Related to Reproduction and Lifespan in <i>Zeugodacus cucurbitae<\/i> (Coquillett) Under Extreme Heat Conditions"],"prefix":"10.1002","volume":"117","author":[{"given":"Yuyang","family":"Lian","sequence":"first","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Medicine Chongqing University Chongqing China"},{"name":"State Key Laboratory of Resource Insects, Institute of Apicultural Research Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyan","family":"Yang","sequence":"additional","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sihua","family":"Peng","sequence":"additional","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Jia","sequence":"additional","affiliation":[{"name":"Key Laboratory of Plant Disease and Pest Control of Hainan Province\/Institute of Plant Protection Hainan Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences) Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejie","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Tropical Fruit Tree Biology of Hainan Province Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianxing","family":"Wu","sequence":"additional","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Yang","sequence":"additional","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5644-6998","authenticated-orcid":false,"given":"Shihao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Sanya Nanfan Research Institute of Hainan University Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2011.09.004"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/npre.2010.4282.1"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru489"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2015.02.012"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.131205"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects14020189"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2007.10.003"},{"key":"e_1_2_9_10_1","first-page":"1565","article-title":"Effects of Heat Stress on Physiological and Biochemical Mechanisms of Insects: A Literature Review","volume":"4","author":"Du Y.","year":"2007","journal-title":"Acta. 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