{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T22:31:48Z","timestamp":1768775508671,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Zinc homeostasis contributes significantly to numerous physiological processes. <jats:italic>Drosophila<\/jats:italic> ZnT35C protein, a zinc transporter encoded by <jats:italic>CG3994<\/jats:italic>, is chiefly located on the cell membrane and facilitates the transport of zinc from the cytoplasm to the extracellular space to sustain zinc homeostasis within the organism. Previous studies about ZnT35C have involved diverse structures such as the Malpighian tubules, adult brain, and sensory nervous system. Nonetheless, the role of ZnT35C in <jats:italic>Drosophila<\/jats:italic> spermatogenesis remained unclear. In our study, we discovered that ZnT35C plays a pivotal role in <jats:italic>Drosophila<\/jats:italic> spermatogenesis. Its knockdown resulted in sperm loss and male infertility. When <jats:italic>ZnT35C<\/jats:italic> was knocked down in cyst cells, zinc was concentrated within cyst cells, inhibiting the proper development of germ cells and thereby causing the incapacity of flies to generate mature sperms. Zinc supplementation can effectively rescue this failure of spermatogenesis. Our research outcomes suggest that ZnT35C, through modulating the zinc environment within the testes, impacts the male fertility of <jats:italic>Drosophila<\/jats:italic>, occupying a crucial position in the spermatogenesis process.<\/jats:p>","DOI":"10.1002\/arch.70017","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T11:18:24Z","timestamp":1737458304000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["ZnT35C Maintains Zinc Homeostasis to Regulate Spermatogenesis in <i>Drosophila<\/i> Testis"],"prefix":"10.1002","volume":"118","author":[{"given":"Jiayu","family":"He","sequence":"first","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Qingdao China"},{"name":"Key Laboratory of Evolution &amp; Marine Biodiversity (Ministry of Education) and Institute of Evolution &amp; Marine Biodiversity Ocean University of China Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Long","family":"Zhao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Evolution &amp; Marine Biodiversity (Ministry of Education) and Institute of Evolution &amp; Marine Biodiversity Ocean University of China Qingdao China"},{"name":"Fisheries College Ocean University of China Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8466-0043","authenticated-orcid":false,"given":"Ying","family":"Su","sequence":"additional","affiliation":[{"name":"College of Marine Life Sciences Ocean University of China Qingdao China"},{"name":"Key Laboratory of Evolution &amp; Marine Biodiversity (Ministry of Education) and Institute of Evolution &amp; Marine Biodiversity Ocean 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