{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:42:01Z","timestamp":1791423721199,"version":"4.3.3"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The Oriental fruit fly, <jats:italic>Bactrocera dorsalis<\/jats:italic> (<jats:italic>B. dorsalis<\/jats:italic>) is a highly invasive, widely distributed notorious pest restricting global fruit trade immensely. There are several approaches to managing this pest, still require newer approaches. In this regard, recently a novel approach called precision\u2010guided sterile insect technique (pgSIT) is gaining momentum in inducing both female sex elimination or sex conversion and male sterility at one go. Developing a species\u2010specific pgSIT system requires validation of targets such as sex determination and spermatogenesis genes. In this regard, <jats:italic>B. dorsalis<\/jats:italic> is highly amenable for area\u2010wide pest management and in the present study, we have validated the loss\u2010of\u2010function of the spermatogenesis\u2010related gene, <jats:italic>tektin1<\/jats:italic> using the CRISPR\/Cas9 ribonucleoprotein (RNP) complex. This gene was cloned from the local isolate of <jats:italic>B. dorsalis<\/jats:italic> and two promising single guide RNAs (sgRNAs) were designed and validated through in vitro restriction analysis. Injection of the RNP complex (sgRNA\u2009+\u2009Cas9 protein) into the G0 embryo resulted in three adult males carrying mutations at the target site. The phenotype of the mutants was determined through crossing studies, namely, \u25b31\u2642 \u00d7 WT \u2640, \u25b32\u2642 \u00d7 WT \u2640, \u25b33\u2642 \u00d7 WT \u2640, and WT \u2642 \u00d7 WT \u2640 and that showed hatching rates of 0%, 11.70%, 0%, and 45.12%, respectively. The mutant males had more nonviable sperm as compared to control. This study underscores the pivotal role of the <jats:italic>Bdtektin1<\/jats:italic> gene for male fertility and is a promising candidate for further development of pgSIT system for <jats:italic>B. dorsalis<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/arch.70043","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T09:06:08Z","timestamp":1741770368000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["CRISPR\/Cas9 Mediated Editing of <i>Bdtektin1<\/i> Gene Induces Sterility in Male Oriental Fruit Fly, <i>Bactrocera dorsalis<\/i> (Diptera: Tephritidae)"],"prefix":"10.1002","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5462-0588","authenticated-orcid":false,"given":"Chikmagalur Nagaraja","family":"Bhargava","sequence":"first","affiliation":[{"name":"ICAR \u2010 Indian Institute of Horticultural Research Bengaluru Karnataka India"},{"name":"University of Agricultural Sciences Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4094-6285","authenticated-orcid":false,"given":"Karuppannasamy","family":"Ashok","sequence":"additional","affiliation":[{"name":"ICAR \u2010 Indian Institute of Horticultural Research Bengaluru Karnataka India"},{"name":"Tamil Nadu Agricultural University Coimbatore Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7990-9975","authenticated-orcid":false,"given":"Sanjay Kumar","family":"Pradhan","sequence":"additional","affiliation":[{"name":"ICAR \u2010 Indian Institute of Horticultural Research Bengaluru Karnataka India"},{"name":"Hawkesbury Institute for the Environment Western Sydney University Penrith New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sampath","family":"Kumar","sequence":"additional","affiliation":[{"name":"Tata Institute for Genetics and Society Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8541-2247","authenticated-orcid":false,"given":"Maligeppagol","family":"Manamohan","sequence":"additional","affiliation":[{"name":"ICAR \u2010 Indian Institute of Horticultural Research Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Rai","sequence":"additional","affiliation":[{"name":"ICAR \u2010 Indian Agricultural Statistics Research Institute  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0457-8120","authenticated-orcid":false,"given":"Ramasamy","family":"Asokan","sequence":"additional","affiliation":[{"name":"ICAR \u2010 Indian Institute of Horticultural Research Bengaluru Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep01294"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1910(97)00119-4"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1570-7458.2003.00039.x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.25.010180.002013"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007026"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/eea.12180"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/scdb.1998.0227"},{"issue":"6","key":"e_1_2_10_9_1","first-page":"746","article-title":"Screening of Bitter Gourd (Momordica charantia) Germplasm for Sources of Resistance Against Melon Fruit Fly (Bactrocera cucurbitae) in Pakistan","volume":"11","author":"Gogi M. 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