{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T19:26:32Z","timestamp":1777058792322,"version":"3.51.4"},"reference-count":76,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Mirid plant bugs (Hemiptera: Miridae), including <jats:italic>Lygus hesperus<\/jats:italic> (western tarnished plant bug), are key pests of numerous agricultural crops. While management of this pest relies heavily on chemical insecticides, the evolution of resistance and environmental concerns underscore the need for new and more effective approaches. Genetic\u2010based strategies that target male fertiliy are currently being evaluated for population suppression. However, a lack of candidate gene targets with appropriate function, specifically in non\u2010model species like <jats:italic>L. hesperus<\/jats:italic>, has hindered progress in the development and application of such approaches. Given their conserved role in stabilization of the flagella axoneme and association with sperm motility in many organisms, members of the <jats:italic>tektin<\/jats:italic> gene family represent logical targets for genetic\u2010based sterilization. Here, we identified four homologs of the non\u2010vertebrate <jats:italic>tektin<\/jats:italic> family from <jats:italic>L. hesperus<\/jats:italic> and used RNA interference\u2010mediated knockdown to assess their roles in male fertility. Although transcription of the four <jats:italic>tektins<\/jats:italic> was predominantly in the testis, knockdown had negligible effects on either sperm abundance or male fertility. Our results suggest that tektins do not play a critical role in sperm fertilization of eggs in <jats:italic>L<\/jats:italic>. <jats:italic>hesperus<\/jats:italic> and are thus likely poor targets for genetic\u2010based sterilization approaches in this species.<\/jats:p>","DOI":"10.1002\/arch.70053","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T03:38:56Z","timestamp":1743737936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["RNAi\u2010Mediated Knockdown of <i>Tektins<\/i> Does Not Affect Male Fertility in <i>Lygus hesperus<\/i>"],"prefix":"10.1002","volume":"118","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7991-3045","authenticated-orcid":false,"given":"J. 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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roni J.","family":"Gross","sequence":"additional","affiliation":[{"name":"USDA ARS, U.S. Arid Land Agricultural Research Center  Maricopa Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter G.","family":"Fabrick","sequence":"additional","affiliation":[{"name":"USDA ARS, U.S. Arid Land Agricultural Research Center  Maricopa Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"USDA ARS, U.S. Arid Land Agricultural Research Center  Maricopa Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiden M.","family":"Hull","sequence":"additional","affiliation":[{"name":"USDA ARS, U.S. Arid Land Agricultural Research Center  Maricopa Arizona 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