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Calcium is essential for regulating key biological processes in <jats:italic>Bombyx mori<\/jats:italic>, including development, metamorphosis, and reproduction. To explore the role of <jats:italic>BmSERCA<\/jats:italic> in the proliferation of the <jats:italic>B. mori<\/jats:italic> ovary cell line (BmN), intracellular Ca<jats:sup>2+<\/jats:sup> levels were measured following <jats:italic>BmSERCA<\/jats:italic> interference. At 48\u2009h post\u2010interference, intracellular Ca<jats:sup>2+<\/jats:sup> levels increased significantly by 1.38 times. Moreover, transcript levels of genes involved in cell proliferation and division exhibited notable changes. Phenotypic assessments further demonstrated that <jats:italic>BmSERCA<\/jats:italic> interference significantly accelerated the proliferation and migration of BmN cells, with a marked increase in EdU\u2010positive cells. Additionally, flow cytometry analysis revealed that <jats:italic>BmSERCA<\/jats:italic> interference notably affected the cell cycle by inducing G2\/M phase accumulation and elevating the expression of G2\/M phase regulators, including <jats:italic>BmCyclinB<\/jats:italic> and <jats:italic>BmCDK1<\/jats:italic>. Collectively, these findings suggested that <jats:italic>BmSERCA<\/jats:italic> interference disrupted intracellular Ca<jats:sup>2+<\/jats:sup> homeostasis, thereby promoting BmN cell proliferation. This study provided a valuable foundation for understanding the role of <jats:italic>BmSERCA<\/jats:italic> in BmN cell proliferation.<\/jats:p>","DOI":"10.1002\/arch.70079","type":"journal-article","created":{"date-parts":[[2025,7,13]],"date-time":"2025-07-13T19:53:12Z","timestamp":1752436392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mechanisms of <i>BmSERCA<\/i>\u2010Mediated Cell Proliferation Through Ca<sup>2+<\/sup> Homeostasis Regulation"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1635-3571","authenticated-orcid":false,"given":"Xiaoxia","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Sciences Suzhou Medical College of Soochow University Suzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Suzhou Medical College of 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