{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T02:36:44Z","timestamp":1769654204727,"version":"3.49.0"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Helopeltis antonii<\/jats:italic>\n                    Signoret is an important sucking pest of cashew and other tree crops. Reverse transcription\u2010quantitative PCR (RT\u2010qPCR) is a widely used technique for analyzing gene expression. The selection of reliable reference genes is critical for accurate quantification of target gene expression using RT\u2010qPCR. There are no studies to identify reliable reference genes or gene expression studies in\n                    <jats:italic>H. antonii<\/jats:italic>\n                    . In this study, the expression stability of eight candidate genes,\n                    <jats:italic>RPS3A, \u03b2Tubulin1, RPL13A, 18S, EF1\u03b1, Ubiquitin\u2010conjugating enzyme E2 g (UBQ), GAPDH<\/jats:italic>\n                    , and\n                    <jats:italic>TBP<\/jats:italic>\n                    , was evaluated across five different nymphal instars and different adult tissues and sexes of\n                    <jats:italic>H. antonii<\/jats:italic>\n                    . Results of stability analysis using multiple algorithms showed that the expression stability of candidate genes varied across different samples. Comprehensive stability ranking with RefFinder showed that\n                    <jats:italic>RPL13A<\/jats:italic>\n                    and\n                    <jats:italic>TBP<\/jats:italic>\n                    were the most stable in different tissues,\n                    <jats:italic>RPS3A<\/jats:italic>\n                    and\n                    <jats:italic>UBQ<\/jats:italic>\n                    were the most stable in different developmental stages,\n                    <jats:italic>RPS3A<\/jats:italic>\n                    and\n                    <jats:italic>UBQ<\/jats:italic>\n                    were the most stable in the two sexes, and\n                    <jats:italic>UBQ<\/jats:italic>\n                    and\n                    <jats:italic>TUB<\/jats:italic>\n                    were the most stable across all samples of\n                    <jats:italic>H. antonii<\/jats:italic>\n                    . Validation of identified stable reference genes (\n                    <jats:italic>UBQ<\/jats:italic>\n                    and the combination of\n                    <jats:italic>UBQ<\/jats:italic>\n                    and\n                    <jats:italic>TUB<\/jats:italic>\n                    ) in normalization of two target genes (\n                    <jats:italic>COX1<\/jats:italic>\n                    and\n                    <jats:italic>CSP13<\/jats:italic>\n                    ) expression provided consistent and biologically meaningful results. This study represents the first systematic study on the identification of reliable reference genes in\n                    <jats:italic>H. antonii<\/jats:italic>\n                    and provides the basis for future molecular investigations in this important pest species.\n                  <\/jats:p>","DOI":"10.1002\/arch.70125","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T18:32:49Z","timestamp":1769106769000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation and Validation of Stable Reference Genes for Accurate Gene Expression Analysis Across Developmental Stages, Sexes, and Tissues of\n                    <i>Helopeltis antonii<\/i>\n                    (Hemiptera: Miridae)"],"prefix":"10.1002","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3937-6334","authenticated-orcid":false,"given":"Siddanna","family":"Savadi","sequence":"first","affiliation":[{"name":"ICAR\u2010 Directorate of Cashew Research (DCR) Puttur Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manju","family":"Manuel","sequence":"additional","affiliation":[{"name":"ICAR\u2010 Directorate of Cashew Research (DCR) Puttur Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thippagonahally Nagaiya","family":"Raviprasad","sequence":"additional","affiliation":[{"name":"ICAR\u2010 Directorate of Cashew Research (DCR) Puttur Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanitha","family":"Kaliaperumal","sequence":"additional","affiliation":[{"name":"ICAR\u2010 Directorate of Cashew Research (DCR) Puttur Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6182-9339","authenticated-orcid":false,"given":"Rajashekara","family":"Hosalli","sequence":"additional","affiliation":[{"name":"ICAR\u2010 Directorate of Cashew Research (DCR) Puttur Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-501"},{"issue":"4","key":"e_1_2_11_4_1","first-page":"1110","article-title":"Biology of Tea Mosquito Bug, Helopeltis antonii Signoret in Moringa olifera","volume":"50","author":"Aravinthraju K.","year":"2023","journal-title":"Indian Journal of Ecology"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120401"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-25845-3"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2024.101982"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1603\/EC12095"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152730"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/tox253"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes12121982"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00379271.2006.10697472"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0141853"},{"key":"e_1_2_11_15_1","unstructured":"Cristiano G. 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