{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T22:21:25Z","timestamp":1775686885668,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Calnexin (CNX) and calreticulin (CRT) are two essential endoplasmic reticulum chaperones involved in the folding pathway dedicated to N\u2010glycosylated proteins. The N\u2010glycosylated protein GP64, located in the budded virus envelope of\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    nucleopolyhedrovirus (BmNPV), plays a key role in viral entry. However, the roles of CNX and CRT in GP64 processing and maturation, as well as in BmNPV infection, remain incompletely understood. In this study, we identified the two genes\n                    <jats:italic>BmCNX<\/jats:italic>\n                    and\n                    <jats:italic>BmCRT<\/jats:italic>\n                    from a\n                    <jats:italic>B. mori<\/jats:italic>\n                    transcriptome database. Spatiotemporal expression profiles showed that\n                    <jats:italic>BmCNX<\/jats:italic>\n                    and\n                    <jats:italic>BmCRT<\/jats:italic>\n                    displayed similar patterns, with expression detected in all tissues and elevated levels in the gonad, trachea, and malpighian tubule, as well as during the egg and larval stages. Following BmNPV infection, the expression of\n                    <jats:italic>BmCNX<\/jats:italic>\n                    and\n                    <jats:italic>BmCRT<\/jats:italic>\n                    was significantly upregulated in both the midgut and BmN cells. Overexpression of\n                    <jats:italic>BmCNX<\/jats:italic>\n                    and\n                    <jats:italic>BmCRT<\/jats:italic>\n                    enhanced BmNPV proliferation and GP64 expression, while knockdown of either gene suppressed both viral proliferation and GP64 expression. Furthermore, GP64 was shown to interact with BmCNX and BmCRT, respectively. These results suggest that BmNPV infection requires both\n                    <jats:italic>BmCNX<\/jats:italic>\n                    and\n                    <jats:italic>BmCRT<\/jats:italic>\n                    to facilitate GP64 expression and promote viral proliferation. This study lays the foundation for further investigation into the roles of endoplasmic reticulum chaperones in response to BmNPV infection.\n                  <\/jats:p>","DOI":"10.1002\/arch.70129","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T20:06:26Z","timestamp":1770149186000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Bombyx mori<\/i>\n                    ER Chaperones Calnexin and Calreticulin Promote BmNPV Infection Through Facilitating GP64 Expression"],"prefix":"10.1002","volume":"121","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7417-1780","authenticated-orcid":false,"given":"Jie","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Sericulture, Anhui Academy of Agricultural Sciences  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan\u2010shan","family":"Tao","sequence":"additional","affiliation":[{"name":"Institute of Sericulture, Anhui Academy of Agricultural Sciences  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Kong","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong\u2010jun","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Sericulture, Anhui Academy of Agricultural Sciences  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010ping","family":"Xu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Resource Insect Biology and Innovative Utilization, School of Life Sciences Anhui Agricultural University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1483-5730","authenticated-orcid":false,"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Sericulture, Anhui Academy of Agricultural Sciences  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4283-6930","authenticated-orcid":false,"given":"Ming\u2010hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Sericulture, Anhui Academy of Agricultural Sciences  Hefei 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Y.Cao S.Ge A.Xu H.Qian andG.Li.2022. \u201cIdentification of Key Genes Involved in Resistance to Early Stage of BmNPV Infection in Silkworms.\u201d InViruses 2405.","DOI":"10.3390\/v14112405"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.70129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/arch.70129","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.70129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:06:54Z","timestamp":1773029214000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.70129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/arch.70129"],"URL":"https:\/\/doi.org\/10.1002\/arch.70129","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2026-01-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70129"}}