{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T23:53:35Z","timestamp":1774569215541,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    is an important economic insect, and its production efficiency is seriously threatened by B. mori nucleopolyhedrovirus (BmNPV). A deeper understanding of host factors exploited during viral infection is crucial for developing disease\u2010resistant silkworm strains. In the current study, we explored the function of\n                    <jats:italic>Spase12<\/jats:italic>\n                    , a subunit of the signal peptidase complex, in the silkworm's antiviral defense. The tissue expression profile showed that\n                    <jats:italic>Spase12<\/jats:italic>\n                    is primarily expressed in the midgut and ovaries, and its expression was significantly upregulated in BmN cells and midgut tissues following BmNPV infection. Additionally, bioinformatics analyses predicted that\n                    <jats:italic>Spase12<\/jats:italic>\n                    is a two\u2010pass endoplasmic reticulum membrane protein, consistent with its role in protein processing and secretion. Moreover, RNAi\u2010mediated knockdown of\n                    <jats:italic>Spase12<\/jats:italic>\n                    significantly suppressed BmNPV replication, as evidenced by decreased\n                    <jats:italic>VP39<\/jats:italic>\n                    expression. On the other hand, overexpression of Spase12 in BmN cells and in vivo experiments using a recombinant baculovirus system confirmed increased viral proliferation and DNA load in the midgut of infected silkworm larvae. Together, these findings established a pro\u2010viral role for\n                    <jats:italic>Spase12<\/jats:italic>\n                    during BmNPV infection, likely hijacked by the virus to facilitate its replication. This study offers novel insights into host\u2013virus interactions in silkworms and highlights\n                    <jats:italic>Spase12<\/jats:italic>\n                    as a potential molecular target for breeding BmNPV\u2010resistant\n                    <jats:italic>B. mori<\/jats:italic>\n                    strains.\n                  <\/jats:p>","DOI":"10.1002\/arch.70143","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T19:36:02Z","timestamp":1773862562000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Functional Characterization of\n                    <i>Spase12<\/i>\n                    as a Host Factor Contributing to Bombyx mori Nucleopolyhedrovirus Susceptibility in Silkworms"],"prefix":"10.1002","volume":"121","author":[{"given":"Nasir","family":"Mehmood","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Agro\u2010Animal Genomics and Molecular Breeding, Guangdong Engineering Technology Research Center of Sericulture College of Animal Science, South China Agricultural University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wensheng","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Agro\u2010Animal Genomics and Molecular Breeding, Guangdong Engineering Technology Research Center of Sericulture College of Animal Science, South China Agricultural University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wasim","family":"Javaid","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mian Muhammad","family":"Awais","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Agro\u2010Animal Genomics and Molecular Breeding, Guangdong Engineering Technology Research Center of Sericulture College of Animal Science, South China Agricultural University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingchen","family":"Sun","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Agro\u2010Animal Genomics and Molecular Breeding, Guangdong Engineering Technology Research Center of Sericulture College of Animal Science, South China Agricultural University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.67.3.835"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.106.4.1035"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B0-44-451924-6\/00084-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.3.581"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.20.13152"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.28.16460"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01167-14"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47255-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)71541-X"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0060908"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120220-112317"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147423"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.628151"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3925"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.10.006"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M307542200"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)44693-X"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2018.02.012"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.148593"},{"issue":"1","key":"e_1_2_9_22_1","article-title":"Cidofovir 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