{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T23:53:34Z","timestamp":1774569214455,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The initiation of pupal ecdysis behavior by ecdysis\u2010triggering hormone (ETH) has been established in two Lepidopterans and two Dipterans. In Coleoptera\n                    <jats:italic>Leptinotarsa decemlineata<\/jats:italic>\n                    , RNA interference (RNAi) targeting\n                    <jats:italic>eth<\/jats:italic>\n                    impairs pupation. However, whether the RNAi phenotypes result from defects in organ formation or disruption of pupal ecdysis behavior has not been established. Here, we characterized ETH physiological functions during pupation in a potato defoliating ladybird,\n                    <jats:italic>Henosepilachna vigintioctomaculata<\/jats:italic>\n                    .\n                    <jats:italic>Hveth<\/jats:italic>\n                    levels were high just before or right after each molting, it was highly expressed in the trachea\u2010rich tissues, such as dissected epidermis, fat body, and head. RNAi against\n                    <jats:italic>Hveth<\/jats:italic>\n                    impaired pupation of more than 80% of the resultant larvae. They were partially enveloped by the old larval cuticle until death. HE stained slices displayed that new cuticles were well formed under the old ones at the head, pleuron, and pronotum of prothorax. Moreover, adult organs such as compound eyes, mouthparts, and legs were well developed in both the control and the\n                    <jats:italic>Hveth<\/jats:italic>\n                    RNAi groups. Conversely, the freezing sections revealed that no clear separation between the old and the newly formed cuticles in the\n                    <jats:italic>Hveth<\/jats:italic>\n                    RNAi head, in contrast to the control head that showed a visible gap. In addition, the pupal cuticle under the old larval integument was not pigmented. Our findings suggest that ETH initiates pupal ecdysis behavior to shed off old larval exoskeleton and activates pigmentation of pupal cuticle during pupation in\n                    <jats:italic>H. vigintioctomaculata<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/arch.70146","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T19:26:52Z","timestamp":1774294012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ecdysis Triggering Hormone (ETH) Is Vital for Pupation in\n                    <i>Henosepilachna vigintioctomaculata<\/i>"],"prefix":"10.1002","volume":"121","author":[{"given":"Lan\u2010Lan","family":"Liao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University Nanjing 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