{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T17:22:24Z","timestamp":1788542544478,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    An RNA interference (RNAi)\u2010based management strategy has a great potential for control of the tomato leafminer\n                    <jats:italic>Tuta absoluta<\/jats:italic>\n                    , a microlepidoptera pest damaging a broad range of Solanaceae hosts. Nevertheless, whether RNAi efficiency of the target genes is affected by dsRNA reachability and mRNA levels remains to be established in\n                    <jats:italic>T. absoluta<\/jats:italic>\n                    . Two chitin synthase genes,\n                    <jats:italic>TaChSA<\/jats:italic>\n                    and\n                    <jats:italic>TaChSB<\/jats:italic>\n                    , were highly expressed in ectodermally\u2010derived cells and midgut respectively, facilitating to test dsRNA reachability using oral delivery. According to the 2\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>\u0394CT<\/jats:sup>\n                    value,\n                    <jats:italic>TaChSA<\/jats:italic>\n                    average level was much higher than that of\n                    <jats:italic>TaChSB<\/jats:italic>\n                    , allowing us to compare the mRNA levels on RNAi effectiveness. We found that ingestion of bacterially\u2010expressed ds\n                    <jats:italic>ChSA<\/jats:italic>\n                    or ds\n                    <jats:italic>ChSB<\/jats:italic>\n                    for 24\u2009h identically decreased the target mRNA levels in whole body, and in the head, epidermis, foregut and hindgut. Feeding ds\n                    <jats:italic>ChSA<\/jats:italic>\n                    affected larval growth and leaf mining, caused 19.7% larval lethality and 31.6% non\u2010emerged adults. Moreover, RNAi against\n                    <jats:italic>TaChSA<\/jats:italic>\n                    damaged rectum integrity, decreased cuticle thickness, and thinned tracheae. By contrast, ingesting ds\n                    <jats:italic>ChSB<\/jats:italic>\n                    decreased larval weight, led to 3.8% larval lethality and 43.2% of resulting pupae failing to emerge. Taken together, ds\n                    <jats:italic>ChSA<\/jats:italic>\n                    and ds\n                    <jats:italic>ChSB<\/jats:italic>\n                    might be systemically transported, and exerted a strong RNAi efficiency irrespective of target mRNA levels. Our results indicate a possible application of bacterially\u2010expressed ds\n                    <jats:italic>ChSA<\/jats:italic>\n                    and ds\n                    <jats:italic>ChSB<\/jats:italic>\n                    in the field to protect growing crops against\n                    <jats:italic>T. absoluta<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/arch.70153","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T17:55:06Z","timestamp":1775670906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["RNAi Targeting\n                    <i>Chitin synthase<\/i>\n                    Genes Specifically Affects Larval Performance in\n                    <i>Tuta absoluta<\/i>"],"prefix":"10.1002","volume":"121","author":[{"given":"Juan","family":"Peng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural 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