{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T11:52:43Z","timestamp":1782301963915,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T00:00:00Z","timestamp":1780790400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["2353057000"],"award-info":[{"award-number":["2353057000"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    CRISPR\/Cas9 gene editing is a transformative tool for genetic studies in non\u2010model organisms like the southern green stinkbug,\n                    <jats:italic>Nezara viridula<\/jats:italic>\n                    . However, current protocols depend on embryonic microinjection of CRISPR\/Cas9, which remains technically difficult. An alternative method of delivering Cas9 ribonucleoprotein directly into female ovaries has been tested in only a few insect species, such as mosquitoes and whiteflies. Here, we developed a simple technique for gene editing by injecting Cas9 ribonucleoprotein into adult\n                    <jats:italic>Nezara viridula<\/jats:italic>\n                    females using ReMOT control or BAPC delivery methods previously described. We observed gene editing of the eye color marker\n                    <jats:italic>white<\/jats:italic>\n                    using the ReMOT method and HhKV ligand. These results demonstrate proof of concept for creating germline mutations in\n                    <jats:italic>N. viridula<\/jats:italic>\n                    . The protocol presented in this study could help advance genetic research in hemipteran pest species.\n                  <\/jats:p>","DOI":"10.1002\/arch.70175","type":"journal-article","created":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T18:15:32Z","timestamp":1780856132000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["CRISPR\/Cas9\u2010Mediated Knockout of\n                    <i>White<\/i>\n                    Gene Produces Eye Color Mutants in the Southern Green Stinkbug,\n                    <i>Nezara viridula<\/i>"],"prefix":"10.1002","volume":"122","author":[{"given":"Karthi","family":"Sengodan","sequence":"first","affiliation":[{"name":"Department of Entomology College of Agriculture, Food and Environment University of Kentucky Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Xian","sequence":"additional","affiliation":[{"name":"Department of Entomology College of Agriculture, Food and Environment University of Kentucky Lexington KY USA"},{"name":"College of Plant Protection Northwest A&amp;F University Yangling China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Shirk","sequence":"additional","affiliation":[{"name":"Entomology &amp; Nematology Department University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff","family":"Howell","sequence":"additional","affiliation":[{"name":"Department of Entomology College of Agriculture, Food and Environment University of Kentucky Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil Kumar","family":"Moola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-3247","authenticated-orcid":false,"given":"Subba Reddy","family":"Palli","sequence":"additional","affiliation":[{"name":"Department of Entomology College of Agriculture, Food and Environment University of Kentucky Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c02653"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.124054"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1235"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects11120838"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05425-9"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12663"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2020.103347"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.113.152710"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2022.07.004"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.13122"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/crispr.2019.0067"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.116.036723"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.166389"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00891"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjab016"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/crispr.2023.0076"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.120.401133"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2024.104232"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.120.303269"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2023.104068"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.103781"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2022.100215"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13355-023-00831-y"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-14620-0"},{"key":"e_1_2_9_26_1","first-page":"1550","article-title":"Receptor\u2010Mediated 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